Abstract Bioluminescence (BL) has revealed an extremely useful analytical tool enabling ultrasensitive detection in biotechnological applications. Following the discovery of luciferin and luciferases, molecular biology techniques allowed the cloning of several luciferases and photoproteins. Among most used BL reporters we find firefly and click-beetle luciferases, bacterial luciferase, Renilla, Gaussia and Cypridina luciferases and calcium-activated photoproteins. According to the specific bioluminescent protein, different substrates and protocols must be applied in the experimental procedure for the bioluminescence measurement. By conjugating (either chemically or by molecular biology techniques) bioluminescent probes to specific targets, it is in fact possible to track a wide range of events and analytes. To aid investigators in the choice and applications of reporter genes, the materials and methods required for BL measurements and experimental protocols are described.
Michelini E., Cevenini L., Mezzanotte L., Roda A. (2009). Luminescent probes and visualization of bioluminescence. NEW YORK : Humana Press - Springer Science [10.1007/978-1-60327-321-3_1].
Luminescent probes and visualization of bioluminescence
MICHELINI, ELISA;CEVENINI, LUCA;MEZZANOTTE, LAURA;RODA, ALDO
2009
Abstract
Abstract Bioluminescence (BL) has revealed an extremely useful analytical tool enabling ultrasensitive detection in biotechnological applications. Following the discovery of luciferin and luciferases, molecular biology techniques allowed the cloning of several luciferases and photoproteins. Among most used BL reporters we find firefly and click-beetle luciferases, bacterial luciferase, Renilla, Gaussia and Cypridina luciferases and calcium-activated photoproteins. According to the specific bioluminescent protein, different substrates and protocols must be applied in the experimental procedure for the bioluminescence measurement. By conjugating (either chemically or by molecular biology techniques) bioluminescent probes to specific targets, it is in fact possible to track a wide range of events and analytes. To aid investigators in the choice and applications of reporter genes, the materials and methods required for BL measurements and experimental protocols are described.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
