Tranexamic acid is a synthetic lysine derivative with antifibrinolytic properties. Precolumn chemical derivatization constitutes an effective technique to enhance its poor detectability. The aim of the present communication is the use of 4,7-phenanthroline-5,6-dione1-3 as labelling reagent for tranexamic acid HPLC analysis by fluorescence and UV-DAD detection. The optimized derivatization reaction was carried out at 68°C for 30 min in presence of phosphate buffer (pH 8) yielding to a highly fluorescent adduct. The chromatographic separations were performed on a Prodigy 5ODS or a Luna Phenyl-Hexyl column according to the fluorimetric or UV-DAD method. The analyses were carried out under isocratic elution conditions using as mobile phase a mixture of triethylammonium phosphate buffer (pH 3)/methanol. The proposed methods were validated and successfully applied to the analysis of pharmaceuticals. The assay results of both methods were statistically compared by means of the Student’s t-test and the variance F-test; no statistically difference was found. 1. Gioia, M. G.; Gatti, R.; Vannini, M.; Hudaib, M.; Chromatographia 2002, 56, 289-294. 2. Gatti, R.; Gioia, M.G.; Di Pietra, A. M.; Anal. Chim. Acta. 2002, 474, 11-20. 3. Gatti, R.; Gioia, M. G.; Biomed. Chromatogr. 2008, 22, 207-213.
R.Gatti, M.G.Gioia (2009). HPLC determination of tranexamic acid in pharmaceuticals by precolumn derivatization. s.l : s.n.
HPLC determination of tranexamic acid in pharmaceuticals by precolumn derivatization
GATTI, RITA;GIOIA, MARIA GRAZIA
2009
Abstract
Tranexamic acid is a synthetic lysine derivative with antifibrinolytic properties. Precolumn chemical derivatization constitutes an effective technique to enhance its poor detectability. The aim of the present communication is the use of 4,7-phenanthroline-5,6-dione1-3 as labelling reagent for tranexamic acid HPLC analysis by fluorescence and UV-DAD detection. The optimized derivatization reaction was carried out at 68°C for 30 min in presence of phosphate buffer (pH 8) yielding to a highly fluorescent adduct. The chromatographic separations were performed on a Prodigy 5ODS or a Luna Phenyl-Hexyl column according to the fluorimetric or UV-DAD method. The analyses were carried out under isocratic elution conditions using as mobile phase a mixture of triethylammonium phosphate buffer (pH 3)/methanol. The proposed methods were validated and successfully applied to the analysis of pharmaceuticals. The assay results of both methods were statistically compared by means of the Student’s t-test and the variance F-test; no statistically difference was found. 1. Gioia, M. G.; Gatti, R.; Vannini, M.; Hudaib, M.; Chromatographia 2002, 56, 289-294. 2. Gatti, R.; Gioia, M.G.; Di Pietra, A. M.; Anal. Chim. Acta. 2002, 474, 11-20. 3. Gatti, R.; Gioia, M. G.; Biomed. Chromatogr. 2008, 22, 207-213.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
