Recent observations in oncologic immunology suggest that the presence of immune complexes (ICs) in serum can be correlated to cancer at early stages [1]. We propose a two-electrodes electrochemical technique (Fig. 1) as a diagnostic tool to detect the presence of ICs in screening tests. The large dimension of the ICs acts as a natural built-in amplifier of the measured interface capacitance, thus allowing for label-free detection. Common instability phenomena have been reduced thanks to the functionalization of the electrodes with a three (ethylene-glycol) alkanethiols self assembled monolayer (SAM) which was designed for surface plasmon resonance (SPR) measurements [2]. Such a SAM is a good passivation layer, as it inhibits redox reactions at the electrodes (Fig. 1) [3]. The device is designed to be easily engineered with standard fabrication techniques, which allows the production of disposable devices.
D. Gazzola, G. Zuccheri, C. Guiducci, A. Ferretti, S. Bonetti, V. Angeletti, et al. (2008). IMMUNE COMPLEX BIOSENSORS DESIGNED FOR CANCER LABEL-FREE SCREENING TESTS. s.l : INSTM.
IMMUNE COMPLEX BIOSENSORS DESIGNED FOR CANCER LABEL-FREE SCREENING TESTS
GAZZOLA, DANIELE;ZUCCHERI, GIAMPAOLO;GUIDUCCI, CARLOTTA;FERRETTI, ANNA;BONETTI, SIMONE;SAMORI', BRUNO
2008
Abstract
Recent observations in oncologic immunology suggest that the presence of immune complexes (ICs) in serum can be correlated to cancer at early stages [1]. We propose a two-electrodes electrochemical technique (Fig. 1) as a diagnostic tool to detect the presence of ICs in screening tests. The large dimension of the ICs acts as a natural built-in amplifier of the measured interface capacitance, thus allowing for label-free detection. Common instability phenomena have been reduced thanks to the functionalization of the electrodes with a three (ethylene-glycol) alkanethiols self assembled monolayer (SAM) which was designed for surface plasmon resonance (SPR) measurements [2]. Such a SAM is a good passivation layer, as it inhibits redox reactions at the electrodes (Fig. 1) [3]. The device is designed to be easily engineered with standard fabrication techniques, which allows the production of disposable devices.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
