Recent observations in oncologic immunology suggest that the presence of immune complexes (ICs) in serum can be correlated to cancer at early stages [1]. We propose a two-electrodes electrochemical technique (Fig. 1) as a diagnostic tool to detect the presence of ICs in screening tests. The large dimension of the ICs acts as a natural built-in amplifier of the measured interface capacitance, thus allowing for label-free detection. Common instability phenomena have been reduced thanks to the functionalization of the electrodes with a three (ethylene-glycol) alkanethiols self assembled monolayer (SAM) which was designed for surface plasmon resonance (SPR) measurements [2]. Such a SAM is a good passivation layer, as it inhibits redox reactions at the electrodes (Fig. 1) [3]. The device is designed to be easily engineered with standard fabrication techniques, which allows the production of disposable devices.
IMMUNE COMPLEX BIOSENSORS DESIGNED FOR CANCER LABEL-FREE SCREENING TESTS
GAZZOLA, DANIELE;ZUCCHERI, GIAMPAOLO;GUIDUCCI, CARLOTTA;FERRETTI, ANNA;BONETTI, SIMONE;SAMORI', BRUNO
2008
Abstract
Recent observations in oncologic immunology suggest that the presence of immune complexes (ICs) in serum can be correlated to cancer at early stages [1]. We propose a two-electrodes electrochemical technique (Fig. 1) as a diagnostic tool to detect the presence of ICs in screening tests. The large dimension of the ICs acts as a natural built-in amplifier of the measured interface capacitance, thus allowing for label-free detection. Common instability phenomena have been reduced thanks to the functionalization of the electrodes with a three (ethylene-glycol) alkanethiols self assembled monolayer (SAM) which was designed for surface plasmon resonance (SPR) measurements [2]. Such a SAM is a good passivation layer, as it inhibits redox reactions at the electrodes (Fig. 1) [3]. The device is designed to be easily engineered with standard fabrication techniques, which allows the production of disposable devices.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
