Important achievements in the field of layer silicate nanotopography and their interaction with bio-organic molecules are reported. In fact, the ability to control the binding of biological and organic molecules to a crystal surface is central to several research areas and technological applications, in particular biotechnology, catalysis, molecular microarrays, biosensors and environmental sciences. Furthermore, investigating the way mineral surfaces interact with biomolecules has fundamental implications considering that these substrates may have played an important role as catalysts for the polymerization of biomolecules in the prebiotic environment. For example, we found that DNA molecules have different binding affinities and assume different conformations when adsorbed to different layered silicate surfaces. We regularly observed on Chlorite isolated DNA molecules that became stretched bridging adjacent brucite-like surface regions. On these crystals the surface potential anisotropy is able to order and stretch the DNA filament thus inducing a natural change in its conformation. All the experimental results are in good agreement with simulations and mathematical modelling. The active stretching of DNA on Chlorite is a clear indication of the basic and technological potential carried by these minerals when used as substrates for biomolecules.

Nanotopography and surface potential of layered minerals: engineering and biomedical applications

VALDRE', GIOVANNI
2008

Abstract

Important achievements in the field of layer silicate nanotopography and their interaction with bio-organic molecules are reported. In fact, the ability to control the binding of biological and organic molecules to a crystal surface is central to several research areas and technological applications, in particular biotechnology, catalysis, molecular microarrays, biosensors and environmental sciences. Furthermore, investigating the way mineral surfaces interact with biomolecules has fundamental implications considering that these substrates may have played an important role as catalysts for the polymerization of biomolecules in the prebiotic environment. For example, we found that DNA molecules have different binding affinities and assume different conformations when adsorbed to different layered silicate surfaces. We regularly observed on Chlorite isolated DNA molecules that became stretched bridging adjacent brucite-like surface regions. On these crystals the surface potential anisotropy is able to order and stretch the DNA filament thus inducing a natural change in its conformation. All the experimental results are in good agreement with simulations and mathematical modelling. The active stretching of DNA on Chlorite is a clear indication of the basic and technological potential carried by these minerals when used as substrates for biomolecules.
Interdisciplinary Science and Engineering Materials Research
3
4
G.Valdre
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/70251
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