Phytoplasma detection and identification is primarily based on PCR followed by restriction fragment length polymorphism analysis. This method detects and differentiates phytoplasmas including those not yet identified. The protocol describes the application of this method for identification of phytoplasmas at 16S rRNA (16Sr) group and 16Sr subgroup levels on amplicons and also in silico on the same sequences.
Standard detection protocol: PCR and RFLP analyses based on 16S rRNA gene / Bertaccini A., S. Paltrinieri, N. Contaldo. - STAMPA. - 1875:(2019), pp. 83-95. [10.1007/978-1-4939-8837-2_7]
Standard detection protocol: PCR and RFLP analyses based on 16S rRNA gene
Bertaccini A.
Writing – Review & Editing
;S. PaltrinieriWriting – Original Draft Preparation
;N. ContaldoWriting – Original Draft Preparation
2019
Abstract
Phytoplasma detection and identification is primarily based on PCR followed by restriction fragment length polymorphism analysis. This method detects and differentiates phytoplasmas including those not yet identified. The protocol describes the application of this method for identification of phytoplasmas at 16S rRNA (16Sr) group and 16Sr subgroup levels on amplicons and also in silico on the same sequences.File in questo prodotto:
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