Phytoplasma detection and identification is primarily based on PCR followed by restriction fragment length polymorphism analysis. This method detects and differentiates phytoplasmas including those not yet identified. The protocol describes the application of this method for identification of phytoplasmas at 16S rRNA (16Sr) group and 16Sr subgroup levels on amplicons and also in silico on the same sequences.

Bertaccini A., S.P. (2019). Standard detection protocol: PCR and RFLP analyses based on 16S rRNA gene. New York : Springer Science+Business Media, LLC, part of Springer Nature [10.1007/978-1-4939-8837-2_7].

Standard detection protocol: PCR and RFLP analyses based on 16S rRNA gene

Bertaccini A.
Writing – Review & Editing
;
S. Paltrinieri
Writing – Original Draft Preparation
;
N. Contaldo
Writing – Original Draft Preparation
2019

Abstract

Phytoplasma detection and identification is primarily based on PCR followed by restriction fragment length polymorphism analysis. This method detects and differentiates phytoplasmas including those not yet identified. The protocol describes the application of this method for identification of phytoplasmas at 16S rRNA (16Sr) group and 16Sr subgroup levels on amplicons and also in silico on the same sequences.
2019
Phytoplasmas: Methods and Protocols, Methods in Molecular Biology
83
95
Bertaccini A., S.P. (2019). Standard detection protocol: PCR and RFLP analyses based on 16S rRNA gene. New York : Springer Science+Business Media, LLC, part of Springer Nature [10.1007/978-1-4939-8837-2_7].
Bertaccini A., S. Paltrinieri, N. Contaldo
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/686050
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