Bioluminescence resonance energy transfer (BRET) is a natural phenomenon that has been successfully applied for the study of protein–protein interactions, including opioid receptor oligomers. The discovery of opioid receptor homomers and heteromers has brought to the fi nding of new functions and new way of signaling and traffi cking; therefore, opioid receptor oligomers may be considered as novel drug targets. Fusing receptors of interest with Renilla luciferase and with a fl uorescent protein (such as EYFP), it is possible to study opioid receptor dimerization using BRET.
Baiula, M. (2015). Monitoring opioid receptor dimerization in living cells by bioluminescence resonance energy transfer (BRET). New York, NY : Humana Press [10.1007/978-1-4939-1708-2_8].
Monitoring opioid receptor dimerization in living cells by bioluminescence resonance energy transfer (BRET)
Baiula, Monica
2015
Abstract
Bioluminescence resonance energy transfer (BRET) is a natural phenomenon that has been successfully applied for the study of protein–protein interactions, including opioid receptor oligomers. The discovery of opioid receptor homomers and heteromers has brought to the fi nding of new functions and new way of signaling and traffi cking; therefore, opioid receptor oligomers may be considered as novel drug targets. Fusing receptors of interest with Renilla luciferase and with a fl uorescent protein (such as EYFP), it is possible to study opioid receptor dimerization using BRET.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
