Urine samples collected worldwide for anti-doping testing are not always shipped in refrigerated conditions from collection sites to WADA accredited laboratories, in particular when several transport days are required. Commensal urethral microbiota, urinary pathogens and environmental bacteria may contaminate urine. Enzymatic activity by microbial contamination can cause severe modifications to the excreted compounds, and in particular to doping-relevant peptides such as hormones and growth factors. In recent years, the anti-doping scientific community has also raised strong concerns about the possible use of proteolytic enzymes (proteases) during collection to alter analysis results. Since delays are inevitable and sample refrigeration is not always possible and effective, sound methods for preserving and stabilizing urine samples are desirable. This Project involves the use of Volumetric Absorptive Microsampling (VAMSTM) and Dried Urine Spot (DUS) strategies for the collection of dried microsamples processed by means of streamlined workflows to be developed ad hoc, for high-throughput LC-MS/MS analysis of several peptide hormones and growth factors. DUS and VAMSTM approaches represent promising alternatives to the procedures currently performed by anti-doping laboratories, allowing analyte stabilization by water loss and the consequent broadening of their detection window. This innovative sampling produces logistics savings due to the small transported volume (by air shipments and through customs) and to the possibility to keep the specimens at room temperature, with significant implications on overall analysis cost. The ultimate goal of this project is to establish and validate feasible but reliable protocols for the collection of urine microvolumes, unlikely to be tampered, stably storable and shippable with no particular precautions. The use of innovative microsampling media offers perspectives towards the development of engineered, highly reliable devices, as well as the expansion of their use for the sampling of complementary and promising biological matrices, such as oral fluid.
Laura Mercolini, Michele Protti, Roberto Mandrioli, Paolo Sberna (2019). Enhanced urinary stability and detection window of peptide hormones and growth factors by dried urine microsampling (WADA 2017).
Enhanced urinary stability and detection window of peptide hormones and growth factors by dried urine microsampling (WADA 2017)
Laura Mercolini;Michele Protti;Roberto Mandrioli;
2019
Abstract
Urine samples collected worldwide for anti-doping testing are not always shipped in refrigerated conditions from collection sites to WADA accredited laboratories, in particular when several transport days are required. Commensal urethral microbiota, urinary pathogens and environmental bacteria may contaminate urine. Enzymatic activity by microbial contamination can cause severe modifications to the excreted compounds, and in particular to doping-relevant peptides such as hormones and growth factors. In recent years, the anti-doping scientific community has also raised strong concerns about the possible use of proteolytic enzymes (proteases) during collection to alter analysis results. Since delays are inevitable and sample refrigeration is not always possible and effective, sound methods for preserving and stabilizing urine samples are desirable. This Project involves the use of Volumetric Absorptive Microsampling (VAMSTM) and Dried Urine Spot (DUS) strategies for the collection of dried microsamples processed by means of streamlined workflows to be developed ad hoc, for high-throughput LC-MS/MS analysis of several peptide hormones and growth factors. DUS and VAMSTM approaches represent promising alternatives to the procedures currently performed by anti-doping laboratories, allowing analyte stabilization by water loss and the consequent broadening of their detection window. This innovative sampling produces logistics savings due to the small transported volume (by air shipments and through customs) and to the possibility to keep the specimens at room temperature, with significant implications on overall analysis cost. The ultimate goal of this project is to establish and validate feasible but reliable protocols for the collection of urine microvolumes, unlikely to be tampered, stably storable and shippable with no particular precautions. The use of innovative microsampling media offers perspectives towards the development of engineered, highly reliable devices, as well as the expansion of their use for the sampling of complementary and promising biological matrices, such as oral fluid.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
