Here, we present two approaches to map DNA double-strand breaks (DSBs) and single-strand breaks (SSBs) in the genome of human cells. We named these methods respectively DSB-Seq and SSB-Seq. We tested the DSB and SSB-Seq in HCT1116, human colon cancer cells, and validated the results using the topoisomerase 2 (Top2)-poisoning agent etoposide (ETO). These methods are powerful tools for the direct detection of the physiological and pathological “breakome” of the DNA in human cells.

Mapping DNA breaks by next-generation sequencing

Capranico, Giovanni
Membro del Collaboration Group
;
2018

Abstract

Here, we present two approaches to map DNA double-strand breaks (DSBs) and single-strand breaks (SSBs) in the genome of human cells. We named these methods respectively DSB-Seq and SSB-Seq. We tested the DSB and SSB-Seq in HCT1116, human colon cancer cells, and validated the results using the topoisomerase 2 (Top2)-poisoning agent etoposide (ETO). These methods are powerful tools for the direct detection of the physiological and pathological “breakome” of the DNA in human cells.
2018
Methods in Molecular Biology: Genome Instability
155
166
Baranello, Laura; Kouzine, Fedor; Wojtowicz, Damian; Cui, Kairong; Zhao, Keji; Przytycka, Teresa M.; Capranico, Giovanni; Levens, David
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/625346
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