Here, we present two approaches to map DNA double-strand breaks (DSBs) and single-strand breaks (SSBs) in the genome of human cells. We named these methods respectively DSB-Seq and SSB-Seq. We tested the DSB and SSB-Seq in HCT1116, human colon cancer cells, and validated the results using the topoisomerase 2 (Top2)-poisoning agent etoposide (ETO). These methods are powerful tools for the direct detection of the physiological and pathological âbreakomeâ of the DNA in human cells.
Baranello, L., Kouzine, F., Wojtowicz, D., Cui, K., Zhao, K., Przytycka, T.M., et al. (2018). Mapping DNA breaks by next-generation sequencing. New York, NY : Humana Press Inc. [10.1007/978-1-4939-7306-4_13].
Mapping DNA breaks by next-generation sequencing
Capranico, GiovanniMembro del Collaboration Group
;
2018
Abstract
Here, we present two approaches to map DNA double-strand breaks (DSBs) and single-strand breaks (SSBs) in the genome of human cells. We named these methods respectively DSB-Seq and SSB-Seq. We tested the DSB and SSB-Seq in HCT1116, human colon cancer cells, and validated the results using the topoisomerase 2 (Top2)-poisoning agent etoposide (ETO). These methods are powerful tools for the direct detection of the physiological and pathological âbreakomeâ of the DNA in human cells.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
