In this chapter we illustrate protocols to investigate growth and neurotrophic factors in humans and rodents- (rat and mouse) derived embryonic stem cells (ESC). The conventional two dimensional cell monolayers (2D) system to grow ESC is presented, focusing on the coating strategies also using extracellular matrix components. Then, different approches for 3D stem cell culture are presented, using hydrogels and scaffolds. qPCR, immunocytochemistry, immunenzymatic ELISA assay and multiparametric assays to quantify growth and neurotrophic factor production are presented.

Growth and Neurotrophic Factors in Embryonic Stem Cells

Pannella M
Methodology
;
Giardino L.
Validation
;
Calzà L
Conceptualization
;
Fernández M.
Methodology
2017

Abstract

In this chapter we illustrate protocols to investigate growth and neurotrophic factors in humans and rodents- (rat and mouse) derived embryonic stem cells (ESC). The conventional two dimensional cell monolayers (2D) system to grow ESC is presented, focusing on the coating strategies also using extracellular matrix components. Then, different approches for 3D stem cell culture are presented, using hydrogels and scaffolds. qPCR, immunocytochemistry, immunenzymatic ELISA assay and multiparametric assays to quantify growth and neurotrophic factor production are presented.
Methods in Molecular Biology
272
294
Pannella, M; Giardino, L.; Calzà, L; Fernández, M.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11585/622458
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