The invention relates to thermochemiluminescent silica nanoparticles containing in dispersion in the silica matrix molecules of a thermochemiluminescent acridine-based 1,2-dioxetane and additional chemical species, such as a fluorescent energy acceptor with the function of improving the efficiency of the thermochemiluminescent reaction and/or modifying the emission wavelength, and a method for the synthesis thereof. The nanoparticles are functionalized on the surface with reactive groups (e.g. amines) and procedures are described for bonding them to biospecific probes (e.g. antibodies) and enabling their use as thermochemiluminescent markers in bioanalytical methods (e.g. immunometric methods) based on thermochemi luminescence measurements.
DI FUSCO MASSIMO, ., Guardigli, M., Lombardo, M., Mirasoli, M., Quintavalla, A., Roda, A., et al. (2013). METHOD FOR THE PRODUCTION OF THERMOCHEMILUMINESCENT SILICA NANOPARTICLES AND THEIR USE AS MARKERS IN BIOANALYTIC METHODS.
METHOD FOR THE PRODUCTION OF THERMOCHEMILUMINESCENT SILICA NANOPARTICLES AND THEIR USE AS MARKERS IN BIOANALYTIC METHODS
DI FUSCO MASSIMO;GUARDIGLI MASSIMO;LOMBARDO MARCO;MIRASOLI MARA;QUINTAVALLA ARIANNA;RODA ALDO;TROMBINI CLAUDIO
2013
Abstract
The invention relates to thermochemiluminescent silica nanoparticles containing in dispersion in the silica matrix molecules of a thermochemiluminescent acridine-based 1,2-dioxetane and additional chemical species, such as a fluorescent energy acceptor with the function of improving the efficiency of the thermochemiluminescent reaction and/or modifying the emission wavelength, and a method for the synthesis thereof. The nanoparticles are functionalized on the surface with reactive groups (e.g. amines) and procedures are described for bonding them to biospecific probes (e.g. antibodies) and enabling their use as thermochemiluminescent markers in bioanalytical methods (e.g. immunometric methods) based on thermochemi luminescence measurements.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
