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EnglishHsp70 has been identified in human (Miller et al., Hum Reprod 7:637, 1992) and bovine (Kamaruddin et al., Andrologia 36:327, 2004) ejaculates as well as in extracts from boar sperm (Huang et al., Anim Reprod Sci 63:231, 2000). The aim of this study was to determine Hsp 70 presence and distribution in ejaculated, capacitated and acrosome-reacted boar spermatozoa; the fate of this protein after fertilization was also studied. In fixed-permeabilized fresh semen 92.3±1.8% of cells showed a triangular-shaped immunoreactivity in the equatorial segment of the head. The localization of fluorescent signal underwent several rearrangements after capacitation, becoming particularly evident in the equatorial line in 72.6±5.3% of cells, and in a sub-equatorial band in 88.9±2.3% of sperm after induced acrosome reaction (AR). The proportion of unfixed cells showing the fluorescent signal significantly (P<0.05) increased after AR. No differences in Hsp70 amount between fresh, capacitated and reacted sperm were observed by Western blot. A loss of Hsp immunoreactivity in demembranated spermatozoa was recorded. Perivitelline sperm cells after IVF showed the same pattern as AR-induced cells; after sperm incorporation into the oocyte Hsp positivity became fuzzy and disappeared during sperm decondensation. Our results indicate that Hsp 70 undergoes a redistribution along sperm membrane and is probably translocated from the inner to the outer leaflet after capacitation and acrosome reaction.
| Titolo: | IMMUNOLOCALIZATION OF HEAT SHOCK PROTEIN 70 (Hsp70) IN BOAR SPERMATOZOA |
| Autore/i: | VOLPE, SARA; BERNARDINI, CHIARA; DE AMBROGI, MARCO; TAMANINI, CARLO; SEREN, ERALDO; GALEATI, GIOVANNA; SPINACI, MARCELLA |
| Autore/i Unibo: | |
| Anno: | 2005 |
| Rivista: | |
| Titolo del libro: | Abstracts- 9° ESDAR |
| Pagina iniziale: | 377 |
| Pagina finale: | 377 |
| Abstract: | Hsp70 has been identified in human (Miller et al., Hum Reprod 7:637, 1992) and bovine (Kamaruddin et al., Andrologia 36:327, 2004) ejaculates as well as in extracts from boar sperm (Huang et al., Anim Reprod Sci 63:231, 2000). The aim of this study was to determine Hsp 70 presence and distribution in ejaculated, capacitated and acrosome-reacted boar spermatozoa; the fate of this protein after fertilization was also studied. In fixed-permeabilized fresh semen 92.3±1.8% of cells showed a triangular-shaped immunoreactivity in the equatorial segment of the head. The localization of fluorescent signal underwent several rearrangements after capacitation, becoming particularly evident in the equatorial line in 72.6±5.3% of cells, and in a sub-equatorial band in 88.9±2.3% of sperm after induced acrosome reaction (AR). The proportion of unfixed cells showing the fluorescent signal significantly (P<0.05) increased after AR. No differences in Hsp70 amount between fresh, capacitated and reacted sperm were observed by Western blot. A loss of Hsp immunoreactivity in demembranated spermatozoa was recorded. Perivitelline sperm cells after IVF showed the same pattern as AR-induced cells; after sperm incorporation into the oocyte Hsp positivity became fuzzy and disappeared during sperm decondensation. Our results indicate that Hsp 70 undergoes a redistribution along sperm membrane and is probably translocated from the inner to the outer leaflet after capacitation and acrosome reaction. |
| Data prodotto definitivo in UGOV: | 30-set-2005 |
| Appare nelle tipologie: | 4.02 Riassunto (Abstract) |
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