DETECTION OF BRAF V600E MUTATION BY ALLELE SPECIFIC REAL TIME QUANTITATIVE PCR (AS-QPCR) USING LOCKED NUCLEIC ACIDS PRIMERS AND BEACON PROBES

Method for conducting an allele specific polymerase chain reaction which comprises subjecting DNA which may contain either or both of first and second alleles to a polymerase chain reaction utilizing a primer pair in which one of the primers is complementary to said first allele, but which primer forms a mismatch with said second allele at the 3'-terminal nucleotide of the primer, and utilizing a DNA polymerase wherein said first allele is specifically amplified but no amplification of said second allele occurs.