Detection of kras mutation in exon 2 by allele specific real time quantitative PCR (AS-QPCR)

A method for conducting an allele specific polymerase chain reaction which comprises subjecting DNA which may contain either or both of first and second alleles to a polymerase chain reaction utilizing a primer pair in which one of the primers is complementary to said first allele, but which primer forms a mismatch with said second allele at the 3'-terminal nucleotide of the primer, and utilizing a DNA polymerase wherein said first allele is specifically amplified but no amplification of said second allele occurs.