Tomato spotted wilt virus (TSWV) is the most economically important viral pathogen for both open field and greenhouse ornamental productions in Liguria region. Investigations of the presence and distribution of this Tospovirus in “Piana di Albenga” (Savona) were carried out during 2013 to 2015 including survey and collection of samples belonging to several ornamental species showing symptoms resembling those of TSWV infection. Mechanical inoculations on test plants and serological analysis (PAS-ELISA, LFT) proved TSWV presence in: Alstroemeria spp. (white rings and yellow spots on malformed leaves; asymptomatic flowers), Cyclamen spp. (necrotic concentring rings; malformed flowers), Iberis semperflorens (chloro-necrotic ringspots), Senecio cruentus (necrotic spots), Zinnia elegans (mottling and brown oak-leaf patterns on leaves; colour-breaking on malformed flowers; wilt). Tospoviruses presence was confirmed by RT-PCR using the BR60/BR65 universal primers, which amplify part of the nucleocapsid protein gene of several tospoviruses. Target amplicons of 454 bp were produced for all samples tested. PCR products were cloned and sequenced on both strands. The resulting sequences showed high percentage of identity, ranging from 96.0 to 99.5 %, with several isolates of TSWV. Iberis semperflorens was confirmed as new natural host of TSWV, while the virus was for the first time detected in Z. elegans in Italy. Since TSWV is limiting factor of ornamental plant production and plays important role in the epidemiology of other crops (including aromatic and vegetable species), these results appear serious enough to require better control measures involving both thrips and propagation material.
G. Parrella, L. Piazza, L. Cavicchi, M.G. Bellardi (2015). RECENT FINDINGS OF TOMATO SPOTTED WILT VIRUS INFECTING ORNAMENTAL PLANTS IN LIGURIA.
RECENT FINDINGS OF TOMATO SPOTTED WILT VIRUS INFECTING ORNAMENTAL PLANTS IN LIGURIA
CAVICCHI, LISA;BELLARDI, MARIA GRAZIA
2015
Abstract
Tomato spotted wilt virus (TSWV) is the most economically important viral pathogen for both open field and greenhouse ornamental productions in Liguria region. Investigations of the presence and distribution of this Tospovirus in “Piana di Albenga” (Savona) were carried out during 2013 to 2015 including survey and collection of samples belonging to several ornamental species showing symptoms resembling those of TSWV infection. Mechanical inoculations on test plants and serological analysis (PAS-ELISA, LFT) proved TSWV presence in: Alstroemeria spp. (white rings and yellow spots on malformed leaves; asymptomatic flowers), Cyclamen spp. (necrotic concentring rings; malformed flowers), Iberis semperflorens (chloro-necrotic ringspots), Senecio cruentus (necrotic spots), Zinnia elegans (mottling and brown oak-leaf patterns on leaves; colour-breaking on malformed flowers; wilt). Tospoviruses presence was confirmed by RT-PCR using the BR60/BR65 universal primers, which amplify part of the nucleocapsid protein gene of several tospoviruses. Target amplicons of 454 bp were produced for all samples tested. PCR products were cloned and sequenced on both strands. The resulting sequences showed high percentage of identity, ranging from 96.0 to 99.5 %, with several isolates of TSWV. Iberis semperflorens was confirmed as new natural host of TSWV, while the virus was for the first time detected in Z. elegans in Italy. Since TSWV is limiting factor of ornamental plant production and plays important role in the epidemiology of other crops (including aromatic and vegetable species), these results appear serious enough to require better control measures involving both thrips and propagation material.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
