In this study the in vitro neutralizing activity of 71 swine sera resulted positive by microimmunofluorescence (MIF) test against Chlamydia suis was evaluated. These sera were collected from swine with clinical signs of conjunctivitis or reproductive disorders, reared in different farms in Emilia Romagna (Northern Italy). Neutralizing antibodies specific for C. suis were present in 57 out of the 71 swine sera tested (80.3%), positive by MIF with titres ranging between 32 and 2048. The 82.8% of the 71 swine sera tested presented by MIF titres ≥ 128. The presence of neutralizing antibodies was significantly correlated (p < 0.001) with MIF titres. All sera (11/11) positive by MIF with titres ranging from 512 and 2048 presented neutralizing antibodies and the 79.2% (42/53) of sera with MIF titres to C. suis ranging from 128 and 256 were neutralizing. Most of the sera presented a complement-dependent neutralizing activity even at a final dilution of 1:40.

In vitro detection of neutralizing antibodies to Chlamydia suis in pig sera.

DONATI, MANUELA;DI FRANCESCO, ANTONIETTA;BALDELLI, RAFFAELLA;PIGNANELLI, SALVATORE;SHURDHI, ALISA;CEVENINI, ROBERTO
2007

Abstract

In this study the in vitro neutralizing activity of 71 swine sera resulted positive by microimmunofluorescence (MIF) test against Chlamydia suis was evaluated. These sera were collected from swine with clinical signs of conjunctivitis or reproductive disorders, reared in different farms in Emilia Romagna (Northern Italy). Neutralizing antibodies specific for C. suis were present in 57 out of the 71 swine sera tested (80.3%), positive by MIF with titres ranging between 32 and 2048. The 82.8% of the 71 swine sera tested presented by MIF titres ≥ 128. The presence of neutralizing antibodies was significantly correlated (p < 0.001) with MIF titres. All sera (11/11) positive by MIF with titres ranging from 512 and 2048 presented neutralizing antibodies and the 79.2% (42/53) of sera with MIF titres to C. suis ranging from 128 and 256 were neutralizing. Most of the sera presented a complement-dependent neutralizing activity even at a final dilution of 1:40.
Proceedings of the 5th Annual Workshop of COST Action 855 Animal Chlamydioses and Zoonotic Implications: “Pathogenesis, epidemiology and zoonotic importance of animal chlamydioses”.
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Donati M.; Di Francesco A.; Baldelli R.; Magnino S.; Pignanelli S.; Shurdhi A.; Delucca F.; Cevenini R.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/46953
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