The aim of this work was to assess the impact of the chronic exposure of CeO2 nanoparticles (NPs) ( 50 to 105 nm nominal size ) on soil microbial biomass. To evaluate if the CeO2 NPs can affect the soil quality, they were mixed to an A1 horizon of Epileptic Cambisols at a concentrations of 100, 500 ppm and incubated in lab for short and medium (7 and 60 days) times, at a constant temperature (25°C) and moisture (60% WHC). The preliminary results of the soil physicochemical analyses have showed an insolubility of the CeO2 NPs at short-term incubation in water, EDTA and aqua regia. The biological assays detect a storing of Ce-CeO2 (0,31 to 49 µg/kg ds) in the microbial biomass at short time that decreases about 25% in the C amount. An increment of the basal respiration and a decrease in the amount of carbon soil microbial biomass determined a higher metabolic quotient (qCO2) than the control test, that identifies a stressful situation, most evident in the short term condition. Physical-chemical characterization of the CeO2 NPs and of the soil before and after the NPs addition, was carried out by means of Environmental Scanning Electron Microscope (ESEM) and an Energy Dispersive Spectroscopy –(EDS) . The investigations showed Ce-NPs and Ce-compounds in both- incubation-condition samples. The control soil showed the presence of cerium associated with other elements, like P, Nd, La, Th e Si. From literature, it appears that these elements identify MonaziteCe/Nb minerals, whose chemical formulas are respectively (Ce, La, Nd, Th) PO4 and (Nd, Ce, La) (P, Si) O4. The presence of CeO2 NPs was clearly detected in soil and recognized by ESEM morphological observations coupled with EDS characterizationThe NPs chemical composition appears unaltered, while the size can be modified by NPs aggregation and clustering. The results contribute to setting reference baseline values of cerium in soil and indicate an impact on the amount of carbon soil microbial biomass due to a higher metabolic quotient (qCO2 ) that can condition the soil fertility.
Serena Carbone, Livia Vittori Antisari, Alberto Fabrizi, Gilmo Vianello, Antonietta Gatti (2011). Response of soil microbial biomass to CeO2 nanoparticles Serena.
Response of soil microbial biomass to CeO2 nanoparticles Serena
CARBONE, SERENA;VITTORI ANTISARI, LIVIA;VIANELLO, GILMO;
2011
Abstract
The aim of this work was to assess the impact of the chronic exposure of CeO2 nanoparticles (NPs) ( 50 to 105 nm nominal size ) on soil microbial biomass. To evaluate if the CeO2 NPs can affect the soil quality, they were mixed to an A1 horizon of Epileptic Cambisols at a concentrations of 100, 500 ppm and incubated in lab for short and medium (7 and 60 days) times, at a constant temperature (25°C) and moisture (60% WHC). The preliminary results of the soil physicochemical analyses have showed an insolubility of the CeO2 NPs at short-term incubation in water, EDTA and aqua regia. The biological assays detect a storing of Ce-CeO2 (0,31 to 49 µg/kg ds) in the microbial biomass at short time that decreases about 25% in the C amount. An increment of the basal respiration and a decrease in the amount of carbon soil microbial biomass determined a higher metabolic quotient (qCO2) than the control test, that identifies a stressful situation, most evident in the short term condition. Physical-chemical characterization of the CeO2 NPs and of the soil before and after the NPs addition, was carried out by means of Environmental Scanning Electron Microscope (ESEM) and an Energy Dispersive Spectroscopy –(EDS) . The investigations showed Ce-NPs and Ce-compounds in both- incubation-condition samples. The control soil showed the presence of cerium associated with other elements, like P, Nd, La, Th e Si. From literature, it appears that these elements identify MonaziteCe/Nb minerals, whose chemical formulas are respectively (Ce, La, Nd, Th) PO4 and (Nd, Ce, La) (P, Si) O4. The presence of CeO2 NPs was clearly detected in soil and recognized by ESEM morphological observations coupled with EDS characterizationThe NPs chemical composition appears unaltered, while the size can be modified by NPs aggregation and clustering. The results contribute to setting reference baseline values of cerium in soil and indicate an impact on the amount of carbon soil microbial biomass due to a higher metabolic quotient (qCO2 ) that can condition the soil fertility.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
