INTRODUCTION Composite scaffolds prepared from natural polymers are expected to have enhanced differentiation properties and as a result they gained much attention in recent years for use in tissue-engineering applications. Although there are various natural polymers available for this purpose, gelatin is commonly studied because of their inherent properties. Gelatin is a denatured collagen and commercially available as biodegradable polymer. It has been exstensively utilized for pharmaceuticaland medical purposes, and its biosafety has been proven through long clinical applications. The main disadvantage of gelatin is due to its poor mechanical properties, which limit its possible applications as a biomaterial. Gelatin materials for long-term biomedical applications must be submitted to crosslinking, which improves both the thermal and the mechanical stability of the material. Genipin is a naturally occurring crosslinking agent, which seems to display promising characteristics. The objectives of this study was to evaluate the effects and the influence of a gelatin /genipin porous composite as scaffold for human osteoblast like cells testing cell viability, adhesion, proliferation and differentiation processes. EXPERIMENTAL METHODS MG63 cells were seeded on gelatin/genepin scaffolds for 7, 14, and 21 days. Cell proliferation assay, light microscopy, transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were carried out to evaluate cell viability, morphological and ultramorphological changes induced by cell/scaffold interaction. RESULTS AND DISCUSSION Cell proliferation assay showed an increase of cells on gelatin scaffold during the experimental time, suggesting the lack of genipin release which in high concentrations could have toxic effects. SEM showed a round shape of MG63 cells on the surface of gelatin scaffold even after 3 days from the seeding, suggesting a decrease on growing rate compared to control samples. The inner structure of the gelatin /genipin sponge is gradually colonized by MG63 cells that appear localized on the big pores of the structure, covering its entire surface. TEM analysis showed that after 20 days of cell/scaffold incubation, cells have changed their shape from a round to a fibroblast like ones, and their cell bodies were strictly connected with the scaffold material. CONCLUSION Our results showed that the porous gelatine/genipin scaffold presented here is an appropriate substrate for cell adhesion, proliferation and differentiation for tissue engineering.

MORPHOLOGICAL EVALUATION OF ADESION AND PROLIFERATION OF MG63 CELLS GROWN ON GELATIN/GENIPIN SCAFFOLD / Mirella Falconi; Adriana Bigi; Roberto Giardino; Milena Fini; Antonio Mazzotti; Gabriella Teti. - ELETTRONICO. - (2012), pp. 73-73. (Intervento presentato al convegno Congresso Nazionale Biomateriali tenutosi a Lecce nel 18-20 giugno 2012).

MORPHOLOGICAL EVALUATION OF ADESION AND PROLIFERATION OF MG63 CELLS GROWN ON GELATIN/GENIPIN SCAFFOLD

FALCONI, MIRELLA;BIGI, ADRIANA;GIARDINO, ROBERTO;FINI, MILENA;MAZZOTTI, ANTONIO;TETI, GABRIELLA
2012

Abstract

INTRODUCTION Composite scaffolds prepared from natural polymers are expected to have enhanced differentiation properties and as a result they gained much attention in recent years for use in tissue-engineering applications. Although there are various natural polymers available for this purpose, gelatin is commonly studied because of their inherent properties. Gelatin is a denatured collagen and commercially available as biodegradable polymer. It has been exstensively utilized for pharmaceuticaland medical purposes, and its biosafety has been proven through long clinical applications. The main disadvantage of gelatin is due to its poor mechanical properties, which limit its possible applications as a biomaterial. Gelatin materials for long-term biomedical applications must be submitted to crosslinking, which improves both the thermal and the mechanical stability of the material. Genipin is a naturally occurring crosslinking agent, which seems to display promising characteristics. The objectives of this study was to evaluate the effects and the influence of a gelatin /genipin porous composite as scaffold for human osteoblast like cells testing cell viability, adhesion, proliferation and differentiation processes. EXPERIMENTAL METHODS MG63 cells were seeded on gelatin/genepin scaffolds for 7, 14, and 21 days. Cell proliferation assay, light microscopy, transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were carried out to evaluate cell viability, morphological and ultramorphological changes induced by cell/scaffold interaction. RESULTS AND DISCUSSION Cell proliferation assay showed an increase of cells on gelatin scaffold during the experimental time, suggesting the lack of genipin release which in high concentrations could have toxic effects. SEM showed a round shape of MG63 cells on the surface of gelatin scaffold even after 3 days from the seeding, suggesting a decrease on growing rate compared to control samples. The inner structure of the gelatin /genipin sponge is gradually colonized by MG63 cells that appear localized on the big pores of the structure, covering its entire surface. TEM analysis showed that after 20 days of cell/scaffold incubation, cells have changed their shape from a round to a fibroblast like ones, and their cell bodies were strictly connected with the scaffold material. CONCLUSION Our results showed that the porous gelatine/genipin scaffold presented here is an appropriate substrate for cell adhesion, proliferation and differentiation for tissue engineering.
2012
SIB Congresso Nazionale Biomateriali
73
73
MORPHOLOGICAL EVALUATION OF ADESION AND PROLIFERATION OF MG63 CELLS GROWN ON GELATIN/GENIPIN SCAFFOLD / Mirella Falconi; Adriana Bigi; Roberto Giardino; Milena Fini; Antonio Mazzotti; Gabriella Teti. - ELETTRONICO. - (2012), pp. 73-73. (Intervento presentato al convegno Congresso Nazionale Biomateriali tenutosi a Lecce nel 18-20 giugno 2012).
Mirella Falconi; Adriana Bigi; Roberto Giardino; Milena Fini; Antonio Mazzotti; Gabriella Teti
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/395148
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