SilvaFeed ENC (S-ENC) is a natural vegetable extract used in the animal feed industry that provides the animal diet with natural elements. Avian reoviruses (ARV) are RNA viruses members of the Orthoreovirus genus, Reoviridae family. ARVs are important causes of disease in poultry, in particular viral arthritis. It is sometimes associated with chronic respiratory diseases and Malabsorption syndrome (MAS) together with different enteric viruses including rotavirus, enterovirus, calicivirus and others. Signs of MAS include anorexia, diarrhoea, dehydration, growth depression and weight loss, which can lead to mortality. Considerable economic losses follow the Malabsorption syndrome. Purpose: Field evidence had previously suggested that this substance could be effective against enteric viral infectious. In this work we evaluated the in vitro activity of S- ENC against ARV. Methods: An ARV strain (R2), isolated in United Kingdom in 1980 was used. The S-ENC antiviral activity against ARV was tested before and after the adsorption of the ARV to VERO cells, in order to evaluate the presence of an intra- or an extra-cellular antiviral activity. The inhibitory effect of S-ENC was expressed as IC50 (concentration of the compound corresponding to 50% inhibition of viral cytopathic effect). The MTT assay has been used to evaluate the 50% cytotoxic concentration (CC50) in the presence of S-ENC on the growth of VERO cells. Results: The CC50 of S-ENC was 243,0 ± 18,4 μg/ml and the IC50 in the intracellular antiviral assay was >200 μg/ml. However S-ENC showed an extracellular antiviral activity IC50 of 54,2 ± 14,4 μg/ml. All results represented the mean ± standard deviation of three independent experiments. Conclusion: S-ENC had in vitro a low cytotoxicity and an extracellular antiviral activity. Therefore these preliminary results suggests that SilvaFeed ENC could be a good means in the control of Malabsorption syndrome. Nevertheless, further in vivo experiments are required to confirm these findings.
In vitro antiviral activity of silvafeed enc against avian reovirus / Lupini C.; Vaccari F.; Cecchinato M.; Galletti E.; Graziani R.; Catelli E.. - ELETTRONICO. - (2006), pp. 104-104. (Intervento presentato al convegno 8th European Congress of Chemotherapy and Infections ECC8. tenutosi a Budapest, Hungary. nel October 25-28 2006).
In vitro antiviral activity of silvafeed enc against avian reovirus.
LUPINI, CATERINA;VACCARI, FRANCESCA;GALLETTI, ELENA;CATELLI, ELENA
2006
Abstract
SilvaFeed ENC (S-ENC) is a natural vegetable extract used in the animal feed industry that provides the animal diet with natural elements. Avian reoviruses (ARV) are RNA viruses members of the Orthoreovirus genus, Reoviridae family. ARVs are important causes of disease in poultry, in particular viral arthritis. It is sometimes associated with chronic respiratory diseases and Malabsorption syndrome (MAS) together with different enteric viruses including rotavirus, enterovirus, calicivirus and others. Signs of MAS include anorexia, diarrhoea, dehydration, growth depression and weight loss, which can lead to mortality. Considerable economic losses follow the Malabsorption syndrome. Purpose: Field evidence had previously suggested that this substance could be effective against enteric viral infectious. In this work we evaluated the in vitro activity of S- ENC against ARV. Methods: An ARV strain (R2), isolated in United Kingdom in 1980 was used. The S-ENC antiviral activity against ARV was tested before and after the adsorption of the ARV to VERO cells, in order to evaluate the presence of an intra- or an extra-cellular antiviral activity. The inhibitory effect of S-ENC was expressed as IC50 (concentration of the compound corresponding to 50% inhibition of viral cytopathic effect). The MTT assay has been used to evaluate the 50% cytotoxic concentration (CC50) in the presence of S-ENC on the growth of VERO cells. Results: The CC50 of S-ENC was 243,0 ± 18,4 μg/ml and the IC50 in the intracellular antiviral assay was >200 μg/ml. However S-ENC showed an extracellular antiviral activity IC50 of 54,2 ± 14,4 μg/ml. All results represented the mean ± standard deviation of three independent experiments. Conclusion: S-ENC had in vitro a low cytotoxicity and an extracellular antiviral activity. Therefore these preliminary results suggests that SilvaFeed ENC could be a good means in the control of Malabsorption syndrome. Nevertheless, further in vivo experiments are required to confirm these findings.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
