The Ventasso Horse (VH) is a native Italian breed selected for saddle, and its name comes from Mount Ventasso, located in the province of Reggio Emilia. According to FAO, the small number of the VH population, of about 300 individuals, place the VH in the list of endangered breeds. During the XX Century, Maremmano and Lipizzan stallions were used to improve VH but no information about the introduction of mares is reported. The sequence analysis of hypervariable region of mitochondrial DNA D-loop is the ideal tools to detect maternal lineages present in this breed today. Samples of 46 VH individuals having different mothers were collected. Total DNA was extracted from hair roots. A 752 nt fragment of the D-loop region was amplified (nucleotides 15,339-16,090 of the reference sequence X79547). The amplified product was sequenced on both strands obtaining a 667 nt sequence (15,372-16,039). The sequences were aligned with MultAlin software. A total of 43 mutation spots, of which 20 showed high frequency, were detected. On the whole, 21 haplotypes were observed. VH sequences were compared with 480 horse mitochondrial D-loop DNA sequences retrieved from GenBank, representing a subset of 1,249 sequences found (March, 2006). Sequences longer than 300 nt where chosen. We also decided to include sequences belonging to all breeds present in the database. Furthermore, an Equus asinus sequence (accession No X97337) was included as outgroup for phylogeny studies. On the whole 99 horse breeds were compared with VH, using a sequence of 254 nt (15,487-15,740) common to all considered samples. With this shorter sequence only 14 out of 20 of the most represented mutation spots were considered. Multiple alignments and phylogeny construction were carried out with Mega 3.1 software. Dendrograms were obtained using Neighbour-Joining procedure with 1,000 bootstrap resampling. This analysis reveals a group of 19 out of 46 VH samples showing an unique haplotype not found in the other sequences considered.
Maternal lineages of Ventasso Horse detected by mitochondrial D-loop sequence analysis / Zambonelli P.; Bigi D.. - ELETTRONICO. - (2006). (Intervento presentato al convegno 30th International Conference of Animal Genetics tenutosi a Porto Seguro, Brazil nel 20-25 agosto 2006).
Maternal lineages of Ventasso Horse detected by mitochondrial D-loop sequence analysis
ZAMBONELLI, PAOLO;BIGI, DANIELE
2006
Abstract
The Ventasso Horse (VH) is a native Italian breed selected for saddle, and its name comes from Mount Ventasso, located in the province of Reggio Emilia. According to FAO, the small number of the VH population, of about 300 individuals, place the VH in the list of endangered breeds. During the XX Century, Maremmano and Lipizzan stallions were used to improve VH but no information about the introduction of mares is reported. The sequence analysis of hypervariable region of mitochondrial DNA D-loop is the ideal tools to detect maternal lineages present in this breed today. Samples of 46 VH individuals having different mothers were collected. Total DNA was extracted from hair roots. A 752 nt fragment of the D-loop region was amplified (nucleotides 15,339-16,090 of the reference sequence X79547). The amplified product was sequenced on both strands obtaining a 667 nt sequence (15,372-16,039). The sequences were aligned with MultAlin software. A total of 43 mutation spots, of which 20 showed high frequency, were detected. On the whole, 21 haplotypes were observed. VH sequences were compared with 480 horse mitochondrial D-loop DNA sequences retrieved from GenBank, representing a subset of 1,249 sequences found (March, 2006). Sequences longer than 300 nt where chosen. We also decided to include sequences belonging to all breeds present in the database. Furthermore, an Equus asinus sequence (accession No X97337) was included as outgroup for phylogeny studies. On the whole 99 horse breeds were compared with VH, using a sequence of 254 nt (15,487-15,740) common to all considered samples. With this shorter sequence only 14 out of 20 of the most represented mutation spots were considered. Multiple alignments and phylogeny construction were carried out with Mega 3.1 software. Dendrograms were obtained using Neighbour-Joining procedure with 1,000 bootstrap resampling. This analysis reveals a group of 19 out of 46 VH samples showing an unique haplotype not found in the other sequences considered.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
