Mussels and other marine organisms secrete protein-based adhesive materials for adherence to substrates. One of the unique structural features of mussel adhesive proteins (MAPs) is the presence of L-3,4-dihydroxyphenylalanine (DOPA), an amino acid that is believed to be responsible for both adhesive and crosslinking characteristics of MAPs. DOPA self-oxidation and polymerisation reactions carried out at a slightly alkaline pH were exploited to coat polymeric scaffolds (electrospun poly(lactic acid-co-glycolic acid), PLGA fibrous matrices) and prostheses (poly(propylene), PP hernia meshes), to confer them the ability to react with proteins for improved cell compatibility, as well as tissue adherence at the implant site. In both cases, fuctionalization parameters, such as DOPA solution concentration, treatment temperature and time, were selected on the basis of an accurate physicochemical (FTIR-ATR, Raman, static contact angle and XPS analyses) and morphological (SEM and AFM analyses) characterisation. DOPA coating was in the form of nano-aggregates on the substrate surface. DOPA-functionalised PP meshes were then coated with a DOPA-functionalised gelatine freeze-dried sponge to obtain a bi-layered prosthesis with improved tissue adherence after implantation. DOPA-functionalised PLGA electrospun membranes (average diameter: 1.37 ± 0.23 µm, apparent porosity: 75.6 ± 1.9 %) were successfully grafted with gelatine to improve cell response.
Biomimetic mussel adhesive protein inspired coatings for biomedical applications
TADDEI, PAOLA;
2014
Abstract
Mussels and other marine organisms secrete protein-based adhesive materials for adherence to substrates. One of the unique structural features of mussel adhesive proteins (MAPs) is the presence of L-3,4-dihydroxyphenylalanine (DOPA), an amino acid that is believed to be responsible for both adhesive and crosslinking characteristics of MAPs. DOPA self-oxidation and polymerisation reactions carried out at a slightly alkaline pH were exploited to coat polymeric scaffolds (electrospun poly(lactic acid-co-glycolic acid), PLGA fibrous matrices) and prostheses (poly(propylene), PP hernia meshes), to confer them the ability to react with proteins for improved cell compatibility, as well as tissue adherence at the implant site. In both cases, fuctionalization parameters, such as DOPA solution concentration, treatment temperature and time, were selected on the basis of an accurate physicochemical (FTIR-ATR, Raman, static contact angle and XPS analyses) and morphological (SEM and AFM analyses) characterisation. DOPA coating was in the form of nano-aggregates on the substrate surface. DOPA-functionalised PP meshes were then coated with a DOPA-functionalised gelatine freeze-dried sponge to obtain a bi-layered prosthesis with improved tissue adherence after implantation. DOPA-functionalised PLGA electrospun membranes (average diameter: 1.37 ± 0.23 µm, apparent porosity: 75.6 ± 1.9 %) were successfully grafted with gelatine to improve cell response.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
