Three different strains of bioluminescent bacteria (Vibrio sp.) have been applied to evaluate the biotoxicity of soil samples from oil terminals (Ben Jabbar and Al Giran, Libya), and to assess the effects of a bioremediation treatment by hydrocarbons degrading bacteria. Toxicity tests based on bioluminescent bacteria (BLB) have been used because of their sensitivity [1-3]. BLB emit light when the environment ensures optimal conditions for all metabolic processes. Then, they are sensitive indicators of xenobiotic biotoxicity because the luminescence decrease in presence of noxious substances reflects the impairment of the cellular functions. We observed that the employed strains showed different sensitivity to the various classes of pollutants. The measurements, carried out in 3% NaCl medium, were done both after short time of samples-BLB contact (60 minutes) and after a longer time interval (16 hours). The results were expressed as percentage of inhibition of blank emission (100%). The information supplied by the acute and chronic tests was in agreement. Analyzing by BLB the hydrocarbons contaminated samples before and after various period of bioremediation treatment it was possible to observe an higher toxicity of the treated samples. The BLB could represent a useful biomonitoring tool even in case of on-field conditions, like for in situ remediation processes. [1] Girotti S, Bolelli L, Roda A, Gentilomi G, Musiani M. (2002). Improved detection of toxic chemicals using bioluminescent bacteria. Anal. Chim Acta 471, 113-120. [2] Girotti S., Bolelli L., Fini F., Monari M., Andreani G., Isani G., Carpené E. (2006). Trace metals in the archid clam Scapharca inaequivalvis: effects of molluscan extract on bioluminescent bacteria. Chemosphere, 65 (4), 627-633. [3] Bolelli L., Bobrovova Z., Ferri E., Fini F., Menotta S., Scandurra S., Fedrizzi G., Girotti S. (2006). Bioluminescent bacteria assay of veterinary drugs in excreta of food-producing animals”. J. Pharm. Biomed. Anal., 42, 88-93.
S.Girotti, M.Piccolo, A.Pompei, D.Matteuzzi, E.Ferri, B.Garcia Morante, et al. (2006). Bioluminescent assays to monitor bioremediation treatments on petroleum contaminated soils. BRASOV : Editura Universitatii Transilvania.
Bioluminescent assays to monitor bioremediation treatments on petroleum contaminated soils
GIROTTI, STEFANO;POMPEI, ANNA;MATTEUZZI, DIEGO;FERRI, ELIDA NORA;BOLELLI, LUCA
2006
Abstract
Three different strains of bioluminescent bacteria (Vibrio sp.) have been applied to evaluate the biotoxicity of soil samples from oil terminals (Ben Jabbar and Al Giran, Libya), and to assess the effects of a bioremediation treatment by hydrocarbons degrading bacteria. Toxicity tests based on bioluminescent bacteria (BLB) have been used because of their sensitivity [1-3]. BLB emit light when the environment ensures optimal conditions for all metabolic processes. Then, they are sensitive indicators of xenobiotic biotoxicity because the luminescence decrease in presence of noxious substances reflects the impairment of the cellular functions. We observed that the employed strains showed different sensitivity to the various classes of pollutants. The measurements, carried out in 3% NaCl medium, were done both after short time of samples-BLB contact (60 minutes) and after a longer time interval (16 hours). The results were expressed as percentage of inhibition of blank emission (100%). The information supplied by the acute and chronic tests was in agreement. Analyzing by BLB the hydrocarbons contaminated samples before and after various period of bioremediation treatment it was possible to observe an higher toxicity of the treated samples. The BLB could represent a useful biomonitoring tool even in case of on-field conditions, like for in situ remediation processes. [1] Girotti S, Bolelli L, Roda A, Gentilomi G, Musiani M. (2002). Improved detection of toxic chemicals using bioluminescent bacteria. Anal. Chim Acta 471, 113-120. [2] Girotti S., Bolelli L., Fini F., Monari M., Andreani G., Isani G., Carpené E. (2006). Trace metals in the archid clam Scapharca inaequivalvis: effects of molluscan extract on bioluminescent bacteria. Chemosphere, 65 (4), 627-633. [3] Bolelli L., Bobrovova Z., Ferri E., Fini F., Menotta S., Scandurra S., Fedrizzi G., Girotti S. (2006). Bioluminescent bacteria assay of veterinary drugs in excreta of food-producing animals”. J. Pharm. Biomed. Anal., 42, 88-93.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
