In the last years in Modena and Reggio Emilia provinces surveys to monitor the spreading of grapevine yellows associated with phytoplasma infection were undertaken. Molecular analysis on the collected samples showed the presence of Flavescence dorée (FD) phytoplasmas, and let to understand that the major threat to these viticultural areas is Bois Noir (BN) that is spreading in an epidemic way (Bondavalli et al., this book). To understand the epidemiological situation of this disease, some of the strongly BN infected vineyards have been taken as a model; plant samples (infected grapevine and weeds) and insects, Hyalesthes obsoletus Signoret, already known as BN vector, and Reptalus panzeri Löw known as a frequent species in vineyards, but not demonstrated to transmit BN phytoplasma, (Palermo et al., 2004), were collected. Nucleic acids were extracted from these materials (Prince et al., 1993; Angelini et al., 2001) and DNA has been used for molecular detection assays (PCR-RFLP) on 16S rRNA gene (Lee et al., 1998). The use of this gene let to identify BN phytoplasmas in many of the grapevine samples (Lambrusco variety), in weeds (Urtica dioica, often asymptomatic, Convolvolus arvensis, Medicago sativa, Galium aparine and Plantago lanceolata) and in insects (H. obsoletus and R. panzeri), and allowed to discriminate a molecular variant in some of the R. panzeri samples; this “16S variant” has been cloned and sequenced, it seems not to have a relevant epidemiological importance since it has never been detected till now in grapevine or weed samples. Samples infected by BN phytoplasmas and by the “16S variant” has been subjected to molecular characterization using tuf gene as a marker (Langer and Maixner, 2004). This further genetic characterization showed the presence of three variants associated to BN in the samples tested: the first (variant D) detected only in the R. panzeri samples infected by the “16S variant”, the second (variant B) detected in grapevine, weeds and insects (H. obsoletus and R. panzeri) and the third (variant A) detected only in grapevine and insects (H. obsoletus and R. panzeri). Except for variant D, that has been detected only in insects samples, variant A resulted as the most frequent in grapevine and H. obsoletus samples collected in BN epidemic areas, but it has never been found in weed samples, neither in nettle or bindweed, that have been reported to be the natural host plants of H. obsoletus. Variant B, detected less frequently in insects and grapevine in BN epidemic contexts, is the only variant found in weed samples in the same areas.

Variabilita’ molecolare di fitoplasmi 16SrXII in vigneti delle province di Modena e Reggio Emilia / Botti S.; S. Paltrinieri; N. Mori; L. Milanesi; R. Bondavalli; A. Bertaccini.. - In: PETRIA. - ISSN 1120-7698. - STAMPA. - 15:(2005), pp. 121-124. (Intervento presentato al convegno terzo inocntro nazionale sulle malattie da fitoplasmi tenutosi a milano nel 22-24 giugno).

Variabilita’ molecolare di fitoplasmi 16SrXII in vigneti delle province di Modena e Reggio Emilia

BOTTI, SIMONA;PALTRINIERI, SAMANTA;BERTACCINI, ASSUNTA
2005

Abstract

In the last years in Modena and Reggio Emilia provinces surveys to monitor the spreading of grapevine yellows associated with phytoplasma infection were undertaken. Molecular analysis on the collected samples showed the presence of Flavescence dorée (FD) phytoplasmas, and let to understand that the major threat to these viticultural areas is Bois Noir (BN) that is spreading in an epidemic way (Bondavalli et al., this book). To understand the epidemiological situation of this disease, some of the strongly BN infected vineyards have been taken as a model; plant samples (infected grapevine and weeds) and insects, Hyalesthes obsoletus Signoret, already known as BN vector, and Reptalus panzeri Löw known as a frequent species in vineyards, but not demonstrated to transmit BN phytoplasma, (Palermo et al., 2004), were collected. Nucleic acids were extracted from these materials (Prince et al., 1993; Angelini et al., 2001) and DNA has been used for molecular detection assays (PCR-RFLP) on 16S rRNA gene (Lee et al., 1998). The use of this gene let to identify BN phytoplasmas in many of the grapevine samples (Lambrusco variety), in weeds (Urtica dioica, often asymptomatic, Convolvolus arvensis, Medicago sativa, Galium aparine and Plantago lanceolata) and in insects (H. obsoletus and R. panzeri), and allowed to discriminate a molecular variant in some of the R. panzeri samples; this “16S variant” has been cloned and sequenced, it seems not to have a relevant epidemiological importance since it has never been detected till now in grapevine or weed samples. Samples infected by BN phytoplasmas and by the “16S variant” has been subjected to molecular characterization using tuf gene as a marker (Langer and Maixner, 2004). This further genetic characterization showed the presence of three variants associated to BN in the samples tested: the first (variant D) detected only in the R. panzeri samples infected by the “16S variant”, the second (variant B) detected in grapevine, weeds and insects (H. obsoletus and R. panzeri) and the third (variant A) detected only in grapevine and insects (H. obsoletus and R. panzeri). Except for variant D, that has been detected only in insects samples, variant A resulted as the most frequent in grapevine and H. obsoletus samples collected in BN epidemic areas, but it has never been found in weed samples, neither in nettle or bindweed, that have been reported to be the natural host plants of H. obsoletus. Variant B, detected less frequently in insects and grapevine in BN epidemic contexts, is the only variant found in weed samples in the same areas.
2005
121
124
Variabilita’ molecolare di fitoplasmi 16SrXII in vigneti delle province di Modena e Reggio Emilia / Botti S.; S. Paltrinieri; N. Mori; L. Milanesi; R. Bondavalli; A. Bertaccini.. - In: PETRIA. - ISSN 1120-7698. - STAMPA. - 15:(2005), pp. 121-124. (Intervento presentato al convegno terzo inocntro nazionale sulle malattie da fitoplasmi tenutosi a milano nel 22-24 giugno).
Botti S.; S. Paltrinieri; N. Mori; L. Milanesi; R. Bondavalli; A. Bertaccini.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/34706
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