Structural and functional molecules (enzymes) at roots of life are proteins, the daily protein intake is basic in the sport world. In the last years food integration with amino acids (AA), mainly branched chain (BCAA), has become of frequent use in the resistance sports too. In fact the BCAA are used by the muscles also for energetic purpose (1,2), therefore BCAA are considered particularly useful during heavy training or race periods. This job wants to analyze the relationships among different AA in the plasma, through some systematic controls executed on cycling national Italian team, during various training periods (aerobic or anaerobic) with BCAA supply. Methods In a period of 7 month, 11 athletes of the national pursuit team were monitored, through 5 gathering and 5 venous blood samples. In the 1st two gathering the job was mostly aerobic. A supply of 0.1 [g]∙per [kg] per day of BCAA was provided. Except the month before the 2nd gathering, when a wash out or control period was performed.In the 3rd gathering the training was mixed: aerobic and anaerobic, while in the last 2 gatherings the training was in track and mostly anaerobic. A 21 AA plasmatic chart has been executed with specific chromatographic techniques (HPLC) on coldly separated plasma. The blood samples were always executed in the morning, under at least 8 hours of food abstinence and after an easy training day. The blood was collected by a 19 gauge needle on Li-Eparine and placed to 4 [°C] Crioplast, centrifuged and the serum was finally frozen to -20 [°C] and analysed. Pearson correlation, cluster and Factorial Analysis have been used to study the relationships among the AA. Results Most elevated correlation coefficients (r) have been found among the BCAA (Leu-Ile .86, Val-Ile .77, Val-Leu .72). r>70 has been found also between: Thr-Ser .85, Thr-Gln .73, Thr-Met .702, Lys-Orn .73. r>60 are numerous (12): 5 of them refers to relationship among BCAA and other AA. Cluster analysis (hierarchical and not) shows 3 defined AA groups: a) BCAA b) Ser, Thr, Gln, Met c) Lys, Orn, Pro, Cys. The Factorial analysis, with Varimax rotation, explains 75% of the variance with 4 factors (Fct): • 1st Fct(25%) introduces elevated saturations with BCAA, Tyr, Ala. • 2nd (20%) explains the variance of Ser, Thr, Gly, Gln, while Met is saturated with all this 2 Fct • 3rd (19%) comprises Lys, Orn, Pro, Cys. • 4th is saturated better with the Phe; while Glu and His are saturates in the 3rd Fct too. Data projection on factorial axis, varying experimental conditions, show that supply gives unlike results in the BCAA than in other AA like Orn, Lys, Cys. The aerobic or anaerobic training brings to unlike answers for Phe goup. References 1. Tipton K.D., Ferrando A.A. et al. (1999). Postexercise net protein synthesis in human muscle from orally administered amino acids. Am J Physiol 276 628-34. 2. Rasmussen B.B., Tipton K.D., et al. (2000). An oral essential aminoacid-carbohydrate supply enhances muscle protein anabolism after resistence exercise. J Appl Physiol 88: 386-92.
Plasmatic amino acidic balance and diet supply during cycling training / Merni F.; Faina M.; Bargossi A.M.; Fiorella P.; Posabella G.. - STAMPA. - (2006), p. 110. (Intervento presentato al convegno 11th annual Congress of the European College of Sport Science tenutosi a Lausanne nel 05-08 July 2006).
Plasmatic amino acidic balance and diet supply during cycling training
MERNI, FRANCO;
2006
Abstract
Structural and functional molecules (enzymes) at roots of life are proteins, the daily protein intake is basic in the sport world. In the last years food integration with amino acids (AA), mainly branched chain (BCAA), has become of frequent use in the resistance sports too. In fact the BCAA are used by the muscles also for energetic purpose (1,2), therefore BCAA are considered particularly useful during heavy training or race periods. This job wants to analyze the relationships among different AA in the plasma, through some systematic controls executed on cycling national Italian team, during various training periods (aerobic or anaerobic) with BCAA supply. Methods In a period of 7 month, 11 athletes of the national pursuit team were monitored, through 5 gathering and 5 venous blood samples. In the 1st two gathering the job was mostly aerobic. A supply of 0.1 [g]∙per [kg] per day of BCAA was provided. Except the month before the 2nd gathering, when a wash out or control period was performed.In the 3rd gathering the training was mixed: aerobic and anaerobic, while in the last 2 gatherings the training was in track and mostly anaerobic. A 21 AA plasmatic chart has been executed with specific chromatographic techniques (HPLC) on coldly separated plasma. The blood samples were always executed in the morning, under at least 8 hours of food abstinence and after an easy training day. The blood was collected by a 19 gauge needle on Li-Eparine and placed to 4 [°C] Crioplast, centrifuged and the serum was finally frozen to -20 [°C] and analysed. Pearson correlation, cluster and Factorial Analysis have been used to study the relationships among the AA. Results Most elevated correlation coefficients (r) have been found among the BCAA (Leu-Ile .86, Val-Ile .77, Val-Leu .72). r>70 has been found also between: Thr-Ser .85, Thr-Gln .73, Thr-Met .702, Lys-Orn .73. r>60 are numerous (12): 5 of them refers to relationship among BCAA and other AA. Cluster analysis (hierarchical and not) shows 3 defined AA groups: a) BCAA b) Ser, Thr, Gln, Met c) Lys, Orn, Pro, Cys. The Factorial analysis, with Varimax rotation, explains 75% of the variance with 4 factors (Fct): • 1st Fct(25%) introduces elevated saturations with BCAA, Tyr, Ala. • 2nd (20%) explains the variance of Ser, Thr, Gly, Gln, while Met is saturated with all this 2 Fct • 3rd (19%) comprises Lys, Orn, Pro, Cys. • 4th is saturated better with the Phe; while Glu and His are saturates in the 3rd Fct too. Data projection on factorial axis, varying experimental conditions, show that supply gives unlike results in the BCAA than in other AA like Orn, Lys, Cys. The aerobic or anaerobic training brings to unlike answers for Phe goup. References 1. Tipton K.D., Ferrando A.A. et al. (1999). Postexercise net protein synthesis in human muscle from orally administered amino acids. Am J Physiol 276 628-34. 2. Rasmussen B.B., Tipton K.D., et al. (2000). An oral essential aminoacid-carbohydrate supply enhances muscle protein anabolism after resistence exercise. J Appl Physiol 88: 386-92.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
