n the frame of the EU FP7 project QBOL the traditional phytoplasma classification based upon molecular phylogeny of the 16S rRNA was employed to generate a barcode system that was implemented by adding two more genes tuf and secA. Sequences from a wide range of phytoplasmas representing the most relevant ‘Candidatus Phytoplasma species’ were generated and deposited in a dedicated database (Q-Bank). Phylogenetic analysis of all the genes used for barcoding has revealed full congruence and concordance with the one obtained by conventional 16S rDNA, indicating that the system can be used for a cost effective and fast phytoplasma identification.
Nicolaisen M., Makarova O., Paltrinieri S., Bertaccini A., Contaldo N. (2014). DNA barcoding of phytoplasmas: a tool for their fast identification. Bologna : IPWG - International Phytoplasmologist Working Group.
DNA barcoding of phytoplasmas: a tool for their fast identification
PALTRINIERI, SAMANTA;BERTACCINI, ASSUNTA;CONTALDO, NICOLETTA
2014
Abstract
n the frame of the EU FP7 project QBOL the traditional phytoplasma classification based upon molecular phylogeny of the 16S rRNA was employed to generate a barcode system that was implemented by adding two more genes tuf and secA. Sequences from a wide range of phytoplasmas representing the most relevant ‘Candidatus Phytoplasma species’ were generated and deposited in a dedicated database (Q-Bank). Phylogenetic analysis of all the genes used for barcoding has revealed full congruence and concordance with the one obtained by conventional 16S rDNA, indicating that the system can be used for a cost effective and fast phytoplasma identification.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
