It is possible to create rigid nanostructures by a careful design of the supramolecular assembly of oligodeoxynucleotides.[1] Using this type of approach, a number of monomeric or polymeric, static or dynamic, nano-objects have assembled in our laboratory. Parallelogram shaped nanostructures made of 4 “four-way junctions” have been assembled in order to make flexible or rigid, linear, branched or circular, supramolecular polymers with a high degree of control. The biochemical and structural characterization of these has been performed using gel electrophoresis and atomic force microscopy. By proper chemical functionalization of the ODNs used for the assembly, it is possible to include non-DNA objects on the structures: this seems a clever strategy towards objects with functional elements located at a controlled distance on a nanostructure, with the possibility of also modulating their dynamics. We have demonstrated this approach by assembling a DNA-based switch that can turn fluorescence on and off.[2] 1. B. Samorì and G. Zuccheri, Angew. Chem. Int. Ed., 2005, 44, 1166-1181; M. Brucale, G. Zuccheri, B. Samorì, Trends in Biotechnology, 2006. 2. M. Brucale, G. Zuccheri, B. Samorì, Org. Biomol. Chem. 2005, 3, 575.

"Supramolecular Polymeric Chains and Dynamic Objects of Nanometric Size Obtained by the Self-Assembly of DNA Oligonucleotides".

ZUCCHERI, GIAMPAOLO;BRUCALE, MARCO;SAMORI', BRUNO
2006

Abstract

It is possible to create rigid nanostructures by a careful design of the supramolecular assembly of oligodeoxynucleotides.[1] Using this type of approach, a number of monomeric or polymeric, static or dynamic, nano-objects have assembled in our laboratory. Parallelogram shaped nanostructures made of 4 “four-way junctions” have been assembled in order to make flexible or rigid, linear, branched or circular, supramolecular polymers with a high degree of control. The biochemical and structural characterization of these has been performed using gel electrophoresis and atomic force microscopy. By proper chemical functionalization of the ODNs used for the assembly, it is possible to include non-DNA objects on the structures: this seems a clever strategy towards objects with functional elements located at a controlled distance on a nanostructure, with the possibility of also modulating their dynamics. We have demonstrated this approach by assembling a DNA-based switch that can turn fluorescence on and off.[2] 1. B. Samorì and G. Zuccheri, Angew. Chem. Int. Ed., 2005, 44, 1166-1181; M. Brucale, G. Zuccheri, B. Samorì, Trends in Biotechnology, 2006. 2. M. Brucale, G. Zuccheri, B. Samorì, Org. Biomol. Chem. 2005, 3, 575.
XXII Congresso Nazionale della Società Chimica Italiana
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G. Zuccheri; M. Brucale; B. Samorì
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11585/30566
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