We have compared fluorescence-detected XANES and EXAFS spectra for the heme Fe atom of purified horse heart cytochrome c, embedded in extensively dehydrated trehalose and sucrose matrices. We have detected significantly different spectra in the trehalose and in the sucrose matrices, indicating a a larger relative dynamic and/or conformational disorder in sucrose. The results support the proposal that the coupling between water-disaccharide matrices and the protein is extremely specific, and much stronger in the case of trehalose.
Matrix effects on the local structure and dynamics of Fe in cytochrome c: a comparison between sucrose and trehalose glassy matrices. SC-2487.
VENTUROLI, GIOVANNI;BOSCHERINI, FEDERICO;FRANCIA, FRANCESCO;VERONESI, GIULIA
2008
Abstract
We have compared fluorescence-detected XANES and EXAFS spectra for the heme Fe atom of purified horse heart cytochrome c, embedded in extensively dehydrated trehalose and sucrose matrices. We have detected significantly different spectra in the trehalose and in the sucrose matrices, indicating a a larger relative dynamic and/or conformational disorder in sucrose. The results support the proposal that the coupling between water-disaccharide matrices and the protein is extremely specific, and much stronger in the case of trehalose.File in questo prodotto:
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