Brown rot caused by Monilinia sp. is one of the most important diseases of stone fruits. In America, South Africa, Australia and New Zeeland, M: fructicola prevails on M. laxa, whereas in Europe M.laxa, M. fructigena are only the species recorded from stone fruits. M. fructicola is a quarantine pahogen. Brown rot causes serious losses in the orchard and during post harvest. In rainy summers, fungicide treatments fail to reduce losses of susceptible cultivars to acceptable levels. Since 2002 M. fructicolawas recorde from France, a survey carried out to check if this pathogen was present also in Emilia Romagna (Northen Italy). Over 60 fungal isolates were obtained from fruits of sweet cherry, apricolt, peach, nectarine, and plum showing brown rot and smoke grey mycelium typical of M. laxa and M. fructicola. Monoconidial isolates were biologically and genetically characterized by PCR analysis. The biological and morphological charcteristics taken into consideraton were mycelial growth rate and appearance, sporulation, length of conidial germ tube, and fungicide resisitance. The biological characters of 40 fungal isolates were also investigated with in vivo and in vitro trials. All isoaltes were identified as M. laxa Significant differences were observed between isolates with respect to growth and sporulation in vitro and in vivo.
Biological and molecular characterization of Monilinia laxa from Emilia Romagna
CASALINI, LUCIA;BARALDI, ELENA;MARI, MARTA
2004
Abstract
Brown rot caused by Monilinia sp. is one of the most important diseases of stone fruits. In America, South Africa, Australia and New Zeeland, M: fructicola prevails on M. laxa, whereas in Europe M.laxa, M. fructigena are only the species recorded from stone fruits. M. fructicola is a quarantine pahogen. Brown rot causes serious losses in the orchard and during post harvest. In rainy summers, fungicide treatments fail to reduce losses of susceptible cultivars to acceptable levels. Since 2002 M. fructicolawas recorde from France, a survey carried out to check if this pathogen was present also in Emilia Romagna (Northen Italy). Over 60 fungal isolates were obtained from fruits of sweet cherry, apricolt, peach, nectarine, and plum showing brown rot and smoke grey mycelium typical of M. laxa and M. fructicola. Monoconidial isolates were biologically and genetically characterized by PCR analysis. The biological and morphological charcteristics taken into consideraton were mycelial growth rate and appearance, sporulation, length of conidial germ tube, and fungicide resisitance. The biological characters of 40 fungal isolates were also investigated with in vivo and in vitro trials. All isoaltes were identified as M. laxa Significant differences were observed between isolates with respect to growth and sporulation in vitro and in vivo.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
