The development of methodology for the chemical synthesis of oligonucleotides is the first step for producing not only the natural G-quadruplex sequences but also a large number of modified G-quadruplexes and other G-based supramolecular motifs; these methodologies will be summarized in this chapter. Natural or chemically modified G-motifs can be characterized by a variety of techniques. This chapter will not give an exhaustive list of methodologies, but simply shows how it is possible to gain structural information on G-quadruplexes by means relatively uncommon techniques such as: (i) differential pulse voltammetry; (ii) atomic force microscopy; (iii) dynamic light scattering; (iv) solution X-ray scattering; (v) temperature gradient gel-electrophoresis; (vi) Langmuir-Blodgett technique. Many other techniques are more usually adopted in G4 characterization (NMR, Scanning Tunnel Microscopy, Circular Dichroism, X-ray diffraction …) and examples of such applications can be found in the contributions to the other chapters of this book.
L. Coga, M. Devetak, S. Masiero, G.P. Spada, I. Drevenšek-Olenik (2013). SPECIFIC BEHAVIOUR OF GUANOSINE IN LIPONUCLEOSIDE THIN FILMS. LONDON : Royal Chemical Society.
SPECIFIC BEHAVIOUR OF GUANOSINE IN LIPONUCLEOSIDE THIN FILMS
MASIERO, STEFANO;SPADA, GIAN PIERO;
2013
Abstract
The development of methodology for the chemical synthesis of oligonucleotides is the first step for producing not only the natural G-quadruplex sequences but also a large number of modified G-quadruplexes and other G-based supramolecular motifs; these methodologies will be summarized in this chapter. Natural or chemically modified G-motifs can be characterized by a variety of techniques. This chapter will not give an exhaustive list of methodologies, but simply shows how it is possible to gain structural information on G-quadruplexes by means relatively uncommon techniques such as: (i) differential pulse voltammetry; (ii) atomic force microscopy; (iii) dynamic light scattering; (iv) solution X-ray scattering; (v) temperature gradient gel-electrophoresis; (vi) Langmuir-Blodgett technique. Many other techniques are more usually adopted in G4 characterization (NMR, Scanning Tunnel Microscopy, Circular Dichroism, X-ray diffraction …) and examples of such applications can be found in the contributions to the other chapters of this book.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.