Atypical adenomatous hyperplasia (AAH) is frequently found in tissue surrounding lung adenocarcinoma and it is considered a precursor of lung cancer. However, little is known about the genetic relationship between lung cancer and the associated foci of AAH. In particular , it is not clear if AAH and adenocarcinoma in the same patients are clonally related each other or represent independent neoplastic foci. To clarify the relationship between AAH and the associated cancer, we studied 23 foci of AAH and 18 adenocarcinomas from 16 patients. Cytologic atypia in each focus of AAH has been graded as slight, moderate or severe. In addition, by microdissection tecnique we obtained DNA from each focus of AAH, each focus of cancer, normal pneumocytes and normal lymphocytes. Therefore we evaluated the genetic distance between AAH and cancer in the same patients, using LOH analysis for 7 microsatellites (D3S1478, D3S1300, D9S942, D17S261, D17S250, D18S46, D19S246) and by direct sequencing of the D-loop region of Mithocondrial DNA. We found that the most frequent LOH in AAH were in D3S1478 and D3S1300 (close to FHIT gene). In addition, the pattern of LOH in AAH foci was different from the pattern of LOH in the cancer from the same patient, indicating that AAH and the associated cancer are genetically indipendent. These results were confirmed also by the phylogenetic trees obtained by direct sequencing of mitochondrial DNA.

Atypical Adenomatous Hyperplasia Of The Lung Is Genetically Indipendent From The Associated Cancer

ASIOLI, SOFIA;MORANDI, LUCA;PESSION, ANNALISA;DAMIANI, STEFANIA
2005

Abstract

Atypical adenomatous hyperplasia (AAH) is frequently found in tissue surrounding lung adenocarcinoma and it is considered a precursor of lung cancer. However, little is known about the genetic relationship between lung cancer and the associated foci of AAH. In particular , it is not clear if AAH and adenocarcinoma in the same patients are clonally related each other or represent independent neoplastic foci. To clarify the relationship between AAH and the associated cancer, we studied 23 foci of AAH and 18 adenocarcinomas from 16 patients. Cytologic atypia in each focus of AAH has been graded as slight, moderate or severe. In addition, by microdissection tecnique we obtained DNA from each focus of AAH, each focus of cancer, normal pneumocytes and normal lymphocytes. Therefore we evaluated the genetic distance between AAH and cancer in the same patients, using LOH analysis for 7 microsatellites (D3S1478, D3S1300, D9S942, D17S261, D17S250, D18S46, D19S246) and by direct sequencing of the D-loop region of Mithocondrial DNA. We found that the most frequent LOH in AAH were in D3S1478 and D3S1300 (close to FHIT gene). In addition, the pattern of LOH in AAH foci was different from the pattern of LOH in the cancer from the same patient, indicating that AAH and the associated cancer are genetically indipendent. These results were confirmed also by the phylogenetic trees obtained by direct sequencing of mitochondrial DNA.
FOURTH BIENNAL SYMPOSIUM PULMONARY PATHOLOGY SOCIETY
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Sofia Asioli; L. Morandi; A. Cavazza; A. Pession; S. Damiani
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/13270
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