The present work has the aim to analyze, for the first time, the physiological activity of the pig’s retina using a standardized human electoretinogram protocol approved by ISCEV. Because of this, 30 healthy females piglets of 3 months old and weighing 30 kg were anesthetized and submitted to a not invasive retinal exam (ERG) with four different light stimulations in order to record the photoreceptors response and to compare the average amplitudes for each protocol with the human and mouse data present in literature. The study confirm that a high cone density and the particular pig’s photoreceptor topography allow this animal to be the better choice for experimental modelling of human cone diseases than mouse. Additionally, this wide data background is a good resource for researchers involved in ophthalmic studies to choose the correct animal model for their experiment and to use the smallest possible number of animals needed to obtain a scientifically valid result.
Scorrano F., Marrocco E., Surace EM., Bacci ML. (2012). STUDY OF THE PORCINE ELECTRORETINOGRAM AS PRECLINICAL MODEL FOR THE TREATMENT OF THE HUMAN RETINA HEREDITARY DEGENERATIONS. SINE LOCO : sine nomine.
STUDY OF THE PORCINE ELECTRORETINOGRAM AS PRECLINICAL MODEL FOR THE TREATMENT OF THE HUMAN RETINA HEREDITARY DEGENERATIONS
SCORRANO, FABRIZIO;BACCI, MARIA LAURA
2012
Abstract
The present work has the aim to analyze, for the first time, the physiological activity of the pig’s retina using a standardized human electoretinogram protocol approved by ISCEV. Because of this, 30 healthy females piglets of 3 months old and weighing 30 kg were anesthetized and submitted to a not invasive retinal exam (ERG) with four different light stimulations in order to record the photoreceptors response and to compare the average amplitudes for each protocol with the human and mouse data present in literature. The study confirm that a high cone density and the particular pig’s photoreceptor topography allow this animal to be the better choice for experimental modelling of human cone diseases than mouse. Additionally, this wide data background is a good resource for researchers involved in ophthalmic studies to choose the correct animal model for their experiment and to use the smallest possible number of animals needed to obtain a scientifically valid result.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
