Analysis of lactose and maltose and their discrimination from other disaccharides such as sucrose is an important and challenging task in food analysis. ESI-MS and MS/MS are a valuable detection methods for underivatized disaccharides, however the absence a distinctive ions and CID fragmentation patterns does not allow discrimination of stereoisomers without good chromatographic resolution. Among the many chromatographic approaches reverse-phase elution on porous graphitic carbon (PGC) appears then most performing and suited to ESI-MS spectrometry. After an overview on LC-MS analysis of oligosaccharides, in this chapter we focus on a recently developed U-HPLC-ESI-MS/MS approach, on PGC columns, able to separate and detect the disaccharides (lactose and maltose) in their anomeric forms, as formate adducts obtained directly in-column. Using a Paul trap to monitor adducts [M+HCOO]- at m/z 387 in ESI negative mode (MS^1) and CID fragment ion [M-H]- at m/z 341 (MS^2), the method offers low pg sensitivity in complex vegetable matrices.

Analysis of Maltose and Lactose by U-HPLC-ESI-MS/MS / L. Valgimigli; S. Gabbanini; R. Matera. - STAMPA. - (2012), pp. 443-463. [10.1039/9781849734929-00443]

Analysis of Maltose and Lactose by U-HPLC-ESI-MS/MS

VALGIMIGLI, LUCA;
2012

Abstract

Analysis of lactose and maltose and their discrimination from other disaccharides such as sucrose is an important and challenging task in food analysis. ESI-MS and MS/MS are a valuable detection methods for underivatized disaccharides, however the absence a distinctive ions and CID fragmentation patterns does not allow discrimination of stereoisomers without good chromatographic resolution. Among the many chromatographic approaches reverse-phase elution on porous graphitic carbon (PGC) appears then most performing and suited to ESI-MS spectrometry. After an overview on LC-MS analysis of oligosaccharides, in this chapter we focus on a recently developed U-HPLC-ESI-MS/MS approach, on PGC columns, able to separate and detect the disaccharides (lactose and maltose) in their anomeric forms, as formate adducts obtained directly in-column. Using a Paul trap to monitor adducts [M+HCOO]- at m/z 387 in ESI negative mode (MS^1) and CID fragment ion [M-H]- at m/z 341 (MS^2), the method offers low pg sensitivity in complex vegetable matrices.
2012
Dietary Sugars: Chemistry, Analysis, Function and Effects
443
463
Analysis of Maltose and Lactose by U-HPLC-ESI-MS/MS / L. Valgimigli; S. Gabbanini; R. Matera. - STAMPA. - (2012), pp. 443-463. [10.1039/9781849734929-00443]
L. Valgimigli; S. Gabbanini; R. Matera
File in questo prodotto:
Eventuali allegati, non sono esposti

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/130009
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? 5
social impact