Detection and identification of phytoplasmas is a laborious process often involving nested PCR followed by restriction enzyme analysis and fine resolution gel electrophoresis. To improve through put, other methods are needed. Microarray technology offers a generic assay that can potentially detect and differentiate all types of phytoplasmas in one assay. The present protocol describes a microarray-based method for identification of phytoplasmas to 16Sr group level.
Microarrays for universal detection and identification of phytoplasmas / Nicolaisen M.; H. Nyskjold; A. Bertaccini. - STAMPA. - (2013), pp. 223-232. [10.1007/978-1-62703-089-2_19]
Microarrays for universal detection and identification of phytoplasmas
BERTACCINI, ASSUNTA
2013
Abstract
Detection and identification of phytoplasmas is a laborious process often involving nested PCR followed by restriction enzyme analysis and fine resolution gel electrophoresis. To improve through put, other methods are needed. Microarray technology offers a generic assay that can potentially detect and differentiate all types of phytoplasmas in one assay. The present protocol describes a microarray-based method for identification of phytoplasmas to 16Sr group level.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
