Detection and identification of phytoplasmas is a laborious process often involving nested PCR followed by restriction enzyme analysis and fine resolution gel electrophoresis. To improve through put, other methods are needed. Microarray technology offers a generic assay that can potentially detect and differentiate all types of phytoplasmas in one assay. The present protocol describes a microarray-based method for identification of phytoplasmas to 16Sr group level.
Microarrays for universal detection and identification of phytoplasmas
BERTACCINI, ASSUNTA
2013
Abstract
Detection and identification of phytoplasmas is a laborious process often involving nested PCR followed by restriction enzyme analysis and fine resolution gel electrophoresis. To improve through put, other methods are needed. Microarray technology offers a generic assay that can potentially detect and differentiate all types of phytoplasmas in one assay. The present protocol describes a microarray-based method for identification of phytoplasmas to 16Sr group level.File in questo prodotto:
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