Aim of this work is to develop an innovative, feasible but reliable qualitative and quantitative analysis of Ketamine and its active metabolite Norketamine in saliva samples, optimizing fast and effective pretreatment procedures and using HPLC coupled to different detection means such as spectrofluorimetry (F) and tandem mass spectrometry (MS/MS). Separation is achieved by means of RP C18 stationary phase and a mobile phase consisting in a mixture of acetonitrile and acidic aqueous buffer. The pretreatment of saliva samples is obtained by a miniaturized system of Micro Extraction by Packed Sorbent (MEPS). The preliminary results are promising and assays are in progress in order to fully validate the method.
Mercolini L., Mandrioli R., Protti M., Tiberi V., Conti M., Ghedini N., et al. (2012). Development of a multidetection HPLC method for the analysis of Ketamine and its active metabolite Norketamine in saliva samples.. ROMA : Società Chimica italiana.
Development of a multidetection HPLC method for the analysis of Ketamine and its active metabolite Norketamine in saliva samples.
MANDRIOLI, ROBERTO;GHEDINI, NADIA;RAGGI, MARIA AUGUSTA
2012
Abstract
Aim of this work is to develop an innovative, feasible but reliable qualitative and quantitative analysis of Ketamine and its active metabolite Norketamine in saliva samples, optimizing fast and effective pretreatment procedures and using HPLC coupled to different detection means such as spectrofluorimetry (F) and tandem mass spectrometry (MS/MS). Separation is achieved by means of RP C18 stationary phase and a mobile phase consisting in a mixture of acetonitrile and acidic aqueous buffer. The pretreatment of saliva samples is obtained by a miniaturized system of Micro Extraction by Packed Sorbent (MEPS). The preliminary results are promising and assays are in progress in order to fully validate the method.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
