We investigated immunohistochemical (IHC) expression of Hsp27, Hsp72, Hsp73 and Hsp90 and their role on overall survival (OS) in 3 normal canine mammary gland (NMG) and 30 mammary tumours (MT) (stages: in situ 10, locally invasive 10, with intravascular emboli 10). Tissues were immunostained using a streptavidin-biotin-peroxidase technique. A semiquantitative (absent, low, intermediate, high) IHC assessment was compared between NMG and MT and among stages by Chi square test. Influence on OS was tested by Log Rank Test. In MT, a significant increase of Hsp27 (P<0.01), Hsp72 (P<0.05) and Hsp90 (P<0.01) and a significant Hsp73 reduction (P<0.01) were observed. Intermediate-high Hsp27 expression was observed only in invasive stages (P<0.01), particularly in cells with squamous metaplasia and infiltrating elements. Hsp72 and Hsp73 immunoreactivity was absent to high in all stages, though intermediate-high pattern was mainly detected in invasive stages, where Hsp72 expression was often associated to tumour necrosis or inflammation. Hsp90 immunolabelling was high in all stages and, like Hsp73, it was strong also in lymphatic emboli. Only Hsp27 was significantly associated to poor prognosis (P<0.01). We conclude that Hsp27, Hsp72 and Hsp90 could be involved in carcinogenesis of canine mammary gland, while only Hsp27 could be indicative of poor outcome.
Heat shock proteins expression in canine malignant mammary tumours
SARLI, GIUSEPPE;
2005
Abstract
We investigated immunohistochemical (IHC) expression of Hsp27, Hsp72, Hsp73 and Hsp90 and their role on overall survival (OS) in 3 normal canine mammary gland (NMG) and 30 mammary tumours (MT) (stages: in situ 10, locally invasive 10, with intravascular emboli 10). Tissues were immunostained using a streptavidin-biotin-peroxidase technique. A semiquantitative (absent, low, intermediate, high) IHC assessment was compared between NMG and MT and among stages by Chi square test. Influence on OS was tested by Log Rank Test. In MT, a significant increase of Hsp27 (P<0.01), Hsp72 (P<0.05) and Hsp90 (P<0.01) and a significant Hsp73 reduction (P<0.01) were observed. Intermediate-high Hsp27 expression was observed only in invasive stages (P<0.01), particularly in cells with squamous metaplasia and infiltrating elements. Hsp72 and Hsp73 immunoreactivity was absent to high in all stages, though intermediate-high pattern was mainly detected in invasive stages, where Hsp72 expression was often associated to tumour necrosis or inflammation. Hsp90 immunolabelling was high in all stages and, like Hsp73, it was strong also in lymphatic emboli. Only Hsp27 was significantly associated to poor prognosis (P<0.01). We conclude that Hsp27, Hsp72 and Hsp90 could be involved in carcinogenesis of canine mammary gland, while only Hsp27 could be indicative of poor outcome.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
