Previous research demonstrated that aflatoxin contamination in corn is reduced by field application of wheat grains pre-inoculated with the non-aflatoxigenic Aspergillus flavus strain NRRL 30797. To facilitate field applications of the biocontrol isolate, a series of laboratory studies were conducted on the reliability and efficiency of replacing wheat grains with the novel bioplastic formulation Mater-Bi(R) to serve as a carrier matrix to formulate this fungus. Mater-Bi(R) granules were inoculated with a conidial suspension of NRRL 30797 to achieve a final cell density of log 7 conidia/granule. Incubation of 20-g soil samples receiving a single Mater-Bi(R) granule for 60-days resulted in log 4.2 to 5.3 propagules of A. flavus/g soil for microbiologically active and sterilized soil, respectively. Increasing the number of granules had no effect on the degree of soil colonization by the biocontrol fungus. In addition to the maintenance of rapid vegetative growth and colonization of soil samples, the bioplastic formulation was highly stable, indicating that Mater-Bi(R) is a suitable substitute for biocontrol applications of A. flavus NRRL 30797.
Granular bioplastic biocontrol composition / ABBAS HAMED K; ACCINELLI CESARE; ZABLOTOWICZ ROBERT M; SACCA MARIA L; WILKINSON JEFF. - (2010).
Granular bioplastic biocontrol composition
ACCINELLI, CESARE;
2010
Abstract
Previous research demonstrated that aflatoxin contamination in corn is reduced by field application of wheat grains pre-inoculated with the non-aflatoxigenic Aspergillus flavus strain NRRL 30797. To facilitate field applications of the biocontrol isolate, a series of laboratory studies were conducted on the reliability and efficiency of replacing wheat grains with the novel bioplastic formulation Mater-Bi(R) to serve as a carrier matrix to formulate this fungus. Mater-Bi(R) granules were inoculated with a conidial suspension of NRRL 30797 to achieve a final cell density of log 7 conidia/granule. Incubation of 20-g soil samples receiving a single Mater-Bi(R) granule for 60-days resulted in log 4.2 to 5.3 propagules of A. flavus/g soil for microbiologically active and sterilized soil, respectively. Increasing the number of granules had no effect on the degree of soil colonization by the biocontrol fungus. In addition to the maintenance of rapid vegetative growth and colonization of soil samples, the bioplastic formulation was highly stable, indicating that Mater-Bi(R) is a suitable substitute for biocontrol applications of A. flavus NRRL 30797.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
