Aim To evaluate the ion-release and the apatite-forming ability (i.e. bioactivity) of a novel, light-curable MTA-containing pulp capping material (TheraCal; Bisco Inc, USA) when immersed in phosphatecontaining solution. Methodology Sample disks (8 mm dia · 1.6 mm) of TheraCal, Dycal and ProRoot MTA (Dentsply, USA) and Vitrebond (3M; USA) were prepared. TheraCal and Vitrebond samples were light-cured on both surfaces for 20 s using a LED light after application of a transparent polyester strip (Directa Matrix Strips; Directa AB, Sweden). Dycal and ProRoot MTA samples were cured at 37°C, 98% relative humidity for a time 50% longer than the final setting time (ISO6876), i.e. 2 min for Dycal, 117 min for ProRoot MTA. The discs were immersed in 10 mL deionized water and calcium and hydroxyl ion release analyzed using ion-selective electrodes after 3 and 24 h and 3, 7, 14, 28 days at 37°C. For bioactivity evaluation, discs were immersed in 10 mL phosphate-containing solution DPBS (Dulbecco’s Phosphate Buffered Saline) at 37°C. The surface chemistry, morphology and formation of apatite on materials surface after 1, 7, 14, 28 days in DPBS was assessed by ESEM-EDX, micro-Raman and FT-IR techniques. Results TheraCal released the most calcium throughout the test period and alkalinized the surrounding fluid initially to pH 10–11 (3 h–3 day) and subsequently to pH 8 (7–28 day). Amorphous apatite (952 cm)1 Raman band) was detected within the first 24 h, while a more crystalline apatite (960 cm)1 Raman band) was seen at 7 days. A layer of apatite entirely covered the surface of TheraCal after 28 days. Conclusions TheraCal and ProRoot MTA demonstrated the capacity to form apatite after just 24 h in DPBS. Dycal showed limited, and Vitrebond no bioactivity. In this laboratory setting, TheraCal acted as a calcium-releasing material that induced the formation of apatite when in contact with biological fluids. It may have potential to promote new dentine formation and cell proliferation.
Gandolfi, M.G., Siboni, F., Taddei, P., B., S., Prati, C. (2011). Calcium and hydroxyl ion release and apatite-precipitation triggering by TheraCal, a new light-curable MTA-containing material for pulp-capping.
Calcium and hydroxyl ion release and apatite-precipitation triggering by TheraCal, a new light-curable MTA-containing material for pulp-capping
GANDOLFI, MARIA GIOVANNA;SIBONI, FRANCESCO;TADDEI, PAOLA;PRATI, CARLO
2011
Abstract
Aim To evaluate the ion-release and the apatite-forming ability (i.e. bioactivity) of a novel, light-curable MTA-containing pulp capping material (TheraCal; Bisco Inc, USA) when immersed in phosphatecontaining solution. Methodology Sample disks (8 mm dia · 1.6 mm) of TheraCal, Dycal and ProRoot MTA (Dentsply, USA) and Vitrebond (3M; USA) were prepared. TheraCal and Vitrebond samples were light-cured on both surfaces for 20 s using a LED light after application of a transparent polyester strip (Directa Matrix Strips; Directa AB, Sweden). Dycal and ProRoot MTA samples were cured at 37°C, 98% relative humidity for a time 50% longer than the final setting time (ISO6876), i.e. 2 min for Dycal, 117 min for ProRoot MTA. The discs were immersed in 10 mL deionized water and calcium and hydroxyl ion release analyzed using ion-selective electrodes after 3 and 24 h and 3, 7, 14, 28 days at 37°C. For bioactivity evaluation, discs were immersed in 10 mL phosphate-containing solution DPBS (Dulbecco’s Phosphate Buffered Saline) at 37°C. The surface chemistry, morphology and formation of apatite on materials surface after 1, 7, 14, 28 days in DPBS was assessed by ESEM-EDX, micro-Raman and FT-IR techniques. Results TheraCal released the most calcium throughout the test period and alkalinized the surrounding fluid initially to pH 10–11 (3 h–3 day) and subsequently to pH 8 (7–28 day). Amorphous apatite (952 cm)1 Raman band) was detected within the first 24 h, while a more crystalline apatite (960 cm)1 Raman band) was seen at 7 days. A layer of apatite entirely covered the surface of TheraCal after 28 days. Conclusions TheraCal and ProRoot MTA demonstrated the capacity to form apatite after just 24 h in DPBS. Dycal showed limited, and Vitrebond no bioactivity. In this laboratory setting, TheraCal acted as a calcium-releasing material that induced the formation of apatite when in contact with biological fluids. It may have potential to promote new dentine formation and cell proliferation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
