Granulosa cells from bovine and porcine ovaries were cultured either in monolayer or in follicle-like barium alginate capsules for 6 days. Morphological investigation by electron scanning microscopy indicated that culture in a three-dimensional (3D) system allows self-organization of spherical–polyhedral shape cells. The luteinization index (progesterone:17-estradiol ratio) was significantly higher for monolayer cells than for the 3D cell culture system, confirming the results of morphological analysis and indicating more physiological growth. The encapsulated 3D culture system appears to be a promising way of obtaining in vitro maturation and development of follicles and oocytes.
Vigo D., Russo V., Faustini M., Stacchezzini S., Conte U., Torre M.L., et al. (2004). Preparation of three-dimensional mammalian ovarian follicular cells and ovarian follicle culture systems in a biocompatible matrix.
Preparation of three-dimensional mammalian ovarian follicular cells and ovarian follicle culture systems in a biocompatible matrix
ACCORSI, PIER ATTILIO;GALEATI, GIOVANNA;SPINACI, MARCELLA
2004
Abstract
Granulosa cells from bovine and porcine ovaries were cultured either in monolayer or in follicle-like barium alginate capsules for 6 days. Morphological investigation by electron scanning microscopy indicated that culture in a three-dimensional (3D) system allows self-organization of spherical–polyhedral shape cells. The luteinization index (progesterone:17-estradiol ratio) was significantly higher for monolayer cells than for the 3D cell culture system, confirming the results of morphological analysis and indicating more physiological growth. The encapsulated 3D culture system appears to be a promising way of obtaining in vitro maturation and development of follicles and oocytes.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
