We present an innovative approach to couple elemental and morphological information from individual cells with high spatial resolution, and apply it to the problem of localization of magnesium and other light elements. The integration of Scanning fluorescence X-ray microscopy (SFXM), and Atomic Force Microscopy provided the relative concentration map of the elements in a single whole dehydrated cell. In this specific experiment spatial resolution of the order of 0.3 μm was obtained, but better resolution is in principle possible. The concentration map shows significant differences with respect to the distribution map, indicating higher Mg concentration in cell periphery.

S. Lagomarsino, S. Iotti, G. Farruggia, A. Cedola, V. Trapani, M. Fratini, et al. (2011). Intracellular concentration map of light elements in whole cells by complementary nano-probe methods. John Libbey Eurotext Ltd.

Intracellular concentration map of light elements in whole cells by complementary nano-probe methods

IOTTI, STEFANO;FARRUGGIA, GIOVANNA;MARRACCINI, CHIARA;
2011

Abstract

We present an innovative approach to couple elemental and morphological information from individual cells with high spatial resolution, and apply it to the problem of localization of magnesium and other light elements. The integration of Scanning fluorescence X-ray microscopy (SFXM), and Atomic Force Microscopy provided the relative concentration map of the elements in a single whole dehydrated cell. In this specific experiment spatial resolution of the order of 0.3 μm was obtained, but better resolution is in principle possible. The concentration map shows significant differences with respect to the distribution map, indicating higher Mg concentration in cell periphery.
2011
European Magnesium Meeting - EUROMAG Bologna 2011. Guest Editors: F.I. Wolf and S. Iotti
145
145
S. Lagomarsino, S. Iotti, G. Farruggia, A. Cedola, V. Trapani, M. Fratini, et al. (2011). Intracellular concentration map of light elements in whole cells by complementary nano-probe methods. John Libbey Eurotext Ltd.
S. Lagomarsino; S. Iotti; G. Farruggia; A. Cedola; V. Trapani; M. Fratini; I. Bukreeva; A. Notargiacomo; L. Mastrototaro; C. Marraccini; A. Sorrentino...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11585/117863
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