We present an innovative approach to couple elemental and morphological information from individual cells with high spatial resolution, and apply it to the problem of localization of magnesium and other light elements. The integration of Scanning fluorescence X-ray microscopy (SFXM), and Atomic Force Microscopy provided the relative concentration map of the elements in a single whole dehydrated cell. In this specific experiment spatial resolution of the order of 0.3 μm was obtained, but better resolution is in principle possible. The concentration map shows significant differences with respect to the distribution map, indicating higher Mg concentration in cell periphery.
Intracellular concentration map of light elements in whole cells by complementary nano-probe methods / S. Lagomarsino; S. Iotti; G. Farruggia; A. Cedola; V. Trapani; M. Fratini; I. Bukreeva; A. Notargiacomo; L. Mastrototaro; C. Marraccini; A. Sorrentino; I. McNulty; S. Vogt; D. Legnini; S. Kim; A. Gianoncelli; J.M. Maier; F.I. Wolf. - In: MAGNESIUM RESEARCH. - ISSN 0953-1424. - STAMPA. - 24(3):(2011), pp. 145-145. (Intervento presentato al convegno II EUROMAG MEETING. BIOLOGY OF MAGNESIUM AND ITS TRANSPORTERS: Implications In Human Diseases tenutosi a Bologna nel 8-11 giugno 2011).
Intracellular concentration map of light elements in whole cells by complementary nano-probe methods
IOTTI, STEFANO;FARRUGGIA, GIOVANNA;MARRACCINI, CHIARA;
2011
Abstract
We present an innovative approach to couple elemental and morphological information from individual cells with high spatial resolution, and apply it to the problem of localization of magnesium and other light elements. The integration of Scanning fluorescence X-ray microscopy (SFXM), and Atomic Force Microscopy provided the relative concentration map of the elements in a single whole dehydrated cell. In this specific experiment spatial resolution of the order of 0.3 μm was obtained, but better resolution is in principle possible. The concentration map shows significant differences with respect to the distribution map, indicating higher Mg concentration in cell periphery.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
