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EnglishRecent studies demonstrated that 2MM-67, a new 3-(5-imidazo[2,1-b]thiazolylmethylene)-2-indolinone, analog of recently published compounds is able to induce apoptotic cell death in HT-29, a colon carcinoma cell line. The cells treated with the proapoptotic agent (500 nM) showed arrest in G2/M phase and increased expression of p21 protein. Moreover it was observed a significant reduction of mitochondrial potential (Δψm), enhanced expression of Bax and, finally, a marked induction of effector caspases. The aim of this work was to assess cellular magnesium levels during 2MM-67-induced apoptosis and if they correlate to Δψm decrease and to an increase in ROS production, characteristic feature of mitochondrion-mediated apoptosis. Intracellular free and total magnesium were evaluated in flow cytometry, respectively by the commercial probe MagFluo4-AM (Kd=4.7 mM), and the new synthesized DCHQ7 (Kd=8.3 μM), while ROS production was assessed by DiClFDA and Dihydrohetidine. Our results indicate that 2MM-67 treatment in HT29 cells can result in rapid declines in intracellular free and total magnesium concomitant with the decrease in mitochondrial membrane potential and the increase of ROS production.
| Titolo: | A new imidazothiazolylmethylen-oxindole induces apoptosis and decline in intracellular free and total magnesium in HT29 colon cancer cells | |
| Autore/i: | CAPPADONE, CONCETTINA; FARRUGGIA, GIOVANNA; MARRACCINI, CHIARA; SGARZI, MASSIMO; LOMBARDO, MARCO; IOTTI, STEFANO | |
| Autore/i Unibo: | ||
| Anno: | 2011 | |
| Rivista: | ||
| Titolo del libro: | European Magnesium Meeting - EUROMAG Bologna 2011. Guest Editors: F.I. Wolf and S. Iotti | |
| Pagina iniziale: | 136 | |
| Pagina finale: | 136 | |
| Abstract: | Recent studies demonstrated that 2MM-67, a new 3-(5-imidazo[2,1-b]thiazolylmethylene)-2-indolinone, analog of recently published compounds is able to induce apoptotic cell death in HT-29, a colon carcinoma cell line. The cells treated with the proapoptotic agent (500 nM) showed arrest in G2/M phase and increased expression of p21 protein. Moreover it was observed a significant reduction of mitochondrial potential (Δψm), enhanced expression of Bax and, finally, a marked induction of effector caspases. The aim of this work was to assess cellular magnesium levels during 2MM-67-induced apoptosis and if they correlate to Δψm decrease and to an increase in ROS production, characteristic feature of mitochondrion-mediated apoptosis. Intracellular free and total magnesium were evaluated in flow cytometry, respectively by the commercial probe MagFluo4-AM (Kd=4.7 mM), and the new synthesized DCHQ7 (Kd=8.3 μM), while ROS production was assessed by DiClFDA and Dihydrohetidine. Our results indicate that 2MM-67 treatment in HT29 cells can result in rapid declines in intracellular free and total magnesium concomitant with the decrease in mitochondrial membrane potential and the increase of ROS production. | |
| Data prodotto definitivo in UGOV: | 25-giu-2013 | |
| Appare nelle tipologie: | 4.02 Riassunto (Abstract) |
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