Standard Biological Parts (pTet-LacI-pLac-LacY-GFP) from the iGEM Registry were used to design a synthetic circuit operated by IPTG as an extracellular stimulus to trigger gene transcription. The circuit was designed to work as a Schmitt Trigger (switch with hysteresis). A genetic program without the LacY gene (open-loop configuration) was first cloned into a high copy number plasmid and was used in E. coli to identify a mathematical model of the molecular circuit. Then, the model was used to predict the behavior in closed-loop and to assess the existence of a switch with hysteresis.
CERONI F., PASINI A., GIORDANO E., CAVALCANTI S. (2008). A Synthetic Genetic Circuit to Implement a Schmitt Trigger in E.coli. s.l : s.n.
A Synthetic Genetic Circuit to Implement a Schmitt Trigger in E.coli
CERONI, FRANCESCA;PASINI, ALICE;GIORDANO, EMANUELE DOMENICO;
2008
Abstract
Standard Biological Parts (pTet-LacI-pLac-LacY-GFP) from the iGEM Registry were used to design a synthetic circuit operated by IPTG as an extracellular stimulus to trigger gene transcription. The circuit was designed to work as a Schmitt Trigger (switch with hysteresis). A genetic program without the LacY gene (open-loop configuration) was first cloned into a high copy number plasmid and was used in E. coli to identify a mathematical model of the molecular circuit. Then, the model was used to predict the behavior in closed-loop and to assess the existence of a switch with hysteresis.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
