Role of aquaporins in the cell signalling mediated by hydrogen peroxide in leukemic cells F. Vieceli Dalla Sega, C. Prata, C. Caliceti, L. Zambonin, D. Fiorentini, G. Hakim, L. Landi Department of Biochemistry “G. Moruzzi”, University of Bologna, Bologna, Italy Aquaporins (AQP) constitute a large family of integral membrane proteins, present in all domains of life, that form pores appearing to be designed for the selective passage of water and glycerol [1]. Hydrogen peroxide has been long thought to be freely diffusible but recent evidence suggest that specific mammalian aquaporin homologues (AQP8) possess the capacity to channel H2O2 across membranes [2,3]. Moreover, it has been recently shown that human cells AQP3 expression can modulate the intracellular level of H2O2 and is required for NOX-derived H2O2 signalling [3]. Redox deregulation of proliferative pathways involving ROS (particularly H2O2) in cancer initiation and progression is now firmly established. Here we tested the hypothesis that specific isoforms of aquaporins regulate the level of intracellular H2O2 produced by NOX affecting downstream pathways linked to cell proliferation. In the present study, we show that in different leukemic cell lines NOX2 and/or NOX4 are expressed and are necessary to produce ROS that, in turn, are able to sustain glucose uptake and cell proliferation. Furthermore, we report that inhibition of aquaporins diminishes intracellular ROS accumulation either when H2O2 is produced by Nox enzymes or when is added exogenously to the medium. Finally, we observe a decrease in glucose transport following aquaporins inhibition. Taken together data suggest that aquaporins can regulate hydrogen peroxide uptake and downstream pathways mediated by H2O2.
F. Vieceli Dalla Sega, C. Prata, C. Caliceti, L. Zambonin, D. Fiorentini, G. Hakim, et al. (2011). Role of aquaporins in the cell signalling mediated by hydrogen peroxide in leukemic cells. Oxford : WILEY-BLACKWELL.
Role of aquaporins in the cell signalling mediated by hydrogen peroxide in leukemic cells
VIECELI DALLA SEGA, FRANCESCO;PRATA, CECILIA;CALICETI, CRISTIANA;ZAMBONIN, LAURA;FIORENTINI, DIANA;
2011
Abstract
Role of aquaporins in the cell signalling mediated by hydrogen peroxide in leukemic cells F. Vieceli Dalla Sega, C. Prata, C. Caliceti, L. Zambonin, D. Fiorentini, G. Hakim, L. Landi Department of Biochemistry “G. Moruzzi”, University of Bologna, Bologna, Italy Aquaporins (AQP) constitute a large family of integral membrane proteins, present in all domains of life, that form pores appearing to be designed for the selective passage of water and glycerol [1]. Hydrogen peroxide has been long thought to be freely diffusible but recent evidence suggest that specific mammalian aquaporin homologues (AQP8) possess the capacity to channel H2O2 across membranes [2,3]. Moreover, it has been recently shown that human cells AQP3 expression can modulate the intracellular level of H2O2 and is required for NOX-derived H2O2 signalling [3]. Redox deregulation of proliferative pathways involving ROS (particularly H2O2) in cancer initiation and progression is now firmly established. Here we tested the hypothesis that specific isoforms of aquaporins regulate the level of intracellular H2O2 produced by NOX affecting downstream pathways linked to cell proliferation. In the present study, we show that in different leukemic cell lines NOX2 and/or NOX4 are expressed and are necessary to produce ROS that, in turn, are able to sustain glucose uptake and cell proliferation. Furthermore, we report that inhibition of aquaporins diminishes intracellular ROS accumulation either when H2O2 is produced by Nox enzymes or when is added exogenously to the medium. Finally, we observe a decrease in glucose transport following aquaporins inhibition. Taken together data suggest that aquaporins can regulate hydrogen peroxide uptake and downstream pathways mediated by H2O2.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
