An ULPC-MS/MS method was optimized in order to measure cobalamins in naturally enriched raw milk and to evaluate their fate during thermal treatments and along the process of cheese making. After addition of methotrexate as internal standard, samples were submitted to heat treatment in presence of cyanide that converts all the less stable cobalamins in cyanocobalamin; then a purification was performed by a solid phase extraction (SPE) step. Reverse phase UPLC separation coupled to tandem mass spectrometry provides a fast and reliable determination.
Zironi E, Gazzotti T, Barbarossa A, Lugoboni B, Pagliuca G (2011). Optimization and validation of an UPLC-MS/MS method for determination of vitamin B12 in milk and dairy products.
Optimization and validation of an UPLC-MS/MS method for determination of vitamin B12 in milk and dairy products
ZIRONI, ELISA;GAZZOTTI, TERESA;BARBAROSSA, ANDREA;LUGOBONI, BARBARA;PAGLIUCA, GIAMPIERO
2011
Abstract
An ULPC-MS/MS method was optimized in order to measure cobalamins in naturally enriched raw milk and to evaluate their fate during thermal treatments and along the process of cheese making. After addition of methotrexate as internal standard, samples were submitted to heat treatment in presence of cyanide that converts all the less stable cobalamins in cyanocobalamin; then a purification was performed by a solid phase extraction (SPE) step. Reverse phase UPLC separation coupled to tandem mass spectrometry provides a fast and reliable determination.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
