We present here a lab-on-a-chip to perform cell centrifugation and re-suspension at single-cell level. Microchannels are used to isolate cells inside microwells where fluid replacement can be obtained thanks to microfluidic mixing. Electrodes integrated into each microwell were used to detect conductivity variation of the infused fluids allowing also for a monitored and automated execution of biological protocols which require supernatant substitution. A protocol involving a precise replacement of the supernatants as required by a cell viability assay was implemented demonstrating single-cell microcentrifugation and re-suspension capabilities as confirmed by calcein uptake shown by fluorescence microscopy.

SINGLE-CELL MICROCENTRIFUGATION WITH MONITORED SUPERNATANT REPLACEMENT IN A MICROWELL BASED DEVICE

FAENZA, ANDREA;BOCCHI, MASSIMO;RAMBELLI, LAURA;GIULIANELLI, LUCA;FRANCHI SCARSELLI, ELEONORA;GUERRIERI, ROBERTO
2011

Abstract

We present here a lab-on-a-chip to perform cell centrifugation and re-suspension at single-cell level. Microchannels are used to isolate cells inside microwells where fluid replacement can be obtained thanks to microfluidic mixing. Electrodes integrated into each microwell were used to detect conductivity variation of the infused fluids allowing also for a monitored and automated execution of biological protocols which require supernatant substitution. A protocol involving a precise replacement of the supernatants as required by a cell viability assay was implemented demonstrating single-cell microcentrifugation and re-suspension capabilities as confirmed by calcein uptake shown by fluorescence microscopy.
Proceedings, MicroTAS 2011 - The 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences
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A. Faenza; M. Bocchi; L. Rambelli; L. Giulianelli; E. Franchi Scarselli; R. Guerrieri
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11585/106951
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