We present here a lab-on-a-chip to perform cell centrifugation and re-suspension at single-cell level. Microchannels are used to isolate cells inside microwells where fluid replacement can be obtained thanks to microfluidic mixing. Electrodes integrated into each microwell were used to detect conductivity variation of the infused fluids allowing also for a monitored and automated execution of biological protocols which require supernatant substitution. A protocol involving a precise replacement of the supernatants as required by a cell viability assay was implemented demonstrating single-cell microcentrifugation and re-suspension capabilities as confirmed by calcein uptake shown by fluorescence microscopy.
A. Faenza, M. Bocchi, L. Rambelli, L. Giulianelli, E. Franchi Scarselli, R. Guerrieri (2011). SINGLE-CELL MICROCENTRIFUGATION WITH MONITORED SUPERNATANT REPLACEMENT IN A MICROWELL BASED DEVICE. s.l : Royal Chemical Society.
SINGLE-CELL MICROCENTRIFUGATION WITH MONITORED SUPERNATANT REPLACEMENT IN A MICROWELL BASED DEVICE
FAENZA, ANDREA;BOCCHI, MASSIMO;RAMBELLI, LAURA;GIULIANELLI, LUCA;FRANCHI SCARSELLI, ELEONORA;GUERRIERI, ROBERTO
2011
Abstract
We present here a lab-on-a-chip to perform cell centrifugation and re-suspension at single-cell level. Microchannels are used to isolate cells inside microwells where fluid replacement can be obtained thanks to microfluidic mixing. Electrodes integrated into each microwell were used to detect conductivity variation of the infused fluids allowing also for a monitored and automated execution of biological protocols which require supernatant substitution. A protocol involving a precise replacement of the supernatants as required by a cell viability assay was implemented demonstrating single-cell microcentrifugation and re-suspension capabilities as confirmed by calcein uptake shown by fluorescence microscopy.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
