We present here an innovative architecture based on negative-dielectrophoresis (nDEP) to perform the controlled isolation and patterning of a programmable number of cells inside microwells. The high efficiency solution we propose features electrodes placed on the floor and sidewalls of the microfluidic channels where microwells are realized and allows for the successful isolation, patterning and live-cell imaging of both single cells and cell clusters, with single cell resolution. Cell viability was assessed after delivery performing a calcein release assay in the microwells.

CONTROLLED ISOLATION AND PATTERNING OF K562 LEUKEMIA CELLS USING ELECTRICALLY ACTIVATED MICROCHANNELS

FAENZA, ANDREA;DUQI, ENRI;PECORARI, NICOLA;RAMBELLI, LAURA;GIULIANELLI, LUCA;LOPEZ, NADIA;BOCCHI, MASSIMO;GUERRIERI, ROBERTO
2011

Abstract

We present here an innovative architecture based on negative-dielectrophoresis (nDEP) to perform the controlled isolation and patterning of a programmable number of cells inside microwells. The high efficiency solution we propose features electrodes placed on the floor and sidewalls of the microfluidic channels where microwells are realized and allows for the successful isolation, patterning and live-cell imaging of both single cells and cell clusters, with single cell resolution. Cell viability was assessed after delivery performing a calcein release assay in the microwells.
Proceedings, The 6th International Conference on Microtechnologies in Medicine and Biology
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A. Faenza; E. Duqi; N. Pecorari; L. Rambelli; L. Giulianelli; N. Lopez; M. Bocchi; R. Guerrieri
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11585/106949
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