We present here an innovative architecture based on negative-dielectrophoresis (nDEP) to perform the controlled isolation and patterning of a programmable number of cells inside microwells. The high efficiency solution we propose features electrodes placed on the floor and sidewalls of the microfluidic channels where microwells are realized and allows for the successful isolation, patterning and live-cell imaging of both single cells and cell clusters, with single cell resolution. Cell viability was assessed after delivery performing a calcein release assay in the microwells.
A. Faenza, E. Duqi, N. Pecorari, L. Rambelli, L. Giulianelli, N. Lopez, et al. (2011). CONTROLLED ISOLATION AND PATTERNING OF K562 LEUKEMIA CELLS USING ELECTRICALLY ACTIVATED MICROCHANNELS. s.l : Royal Chemical Society.
CONTROLLED ISOLATION AND PATTERNING OF K562 LEUKEMIA CELLS USING ELECTRICALLY ACTIVATED MICROCHANNELS
FAENZA, ANDREA;DUQI, ENRI;PECORARI, NICOLA;RAMBELLI, LAURA;GIULIANELLI, LUCA;LOPEZ, NADIA;BOCCHI, MASSIMO;GUERRIERI, ROBERTO
2011
Abstract
We present here an innovative architecture based on negative-dielectrophoresis (nDEP) to perform the controlled isolation and patterning of a programmable number of cells inside microwells. The high efficiency solution we propose features electrodes placed on the floor and sidewalls of the microfluidic channels where microwells are realized and allows for the successful isolation, patterning and live-cell imaging of both single cells and cell clusters, with single cell resolution. Cell viability was assessed after delivery performing a calcein release assay in the microwells.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
