We present the development and application of a microfluidic device that can efficiently and reproducibly isolate a programmable number of cells into microwells. The chip consists of a 3x4 array of 100 µm microwells drilled through a flex-PCB substrate along microchannels. Negative DEP focusing of particles is used to axially centre particles within the channel and also to shield the microwell entrance for flowing particles. An optical detection routine analyses and selects the cells to be delivered with single cell resolution. Cell viability results based on a calcein release assay performed on single cells are within the range of physiological loss of fluorescence intensity.
E. Duqi, A. Faenza, L. Giulianelli, N. Pecorari, L. Rambelli, N. Lopez, et al. (2011). AUTOMATED ISOLATION OF A PROGRAMMABLE NUMBER OF CELLS INTO MICROWELLS USING DEP FORCES AND OPTICAL DETECTION. s.l : s.n.
AUTOMATED ISOLATION OF A PROGRAMMABLE NUMBER OF CELLS INTO MICROWELLS USING DEP FORCES AND OPTICAL DETECTION
DUQI, ENRI;FAENZA, ANDREA;GIULIANELLI, LUCA;PECORARI, NICOLA;RAMBELLI, LAURA;LOPEZ, NADIA;BOCCHI, MASSIMO;FRANCHI SCARSELLI, ELEONORA;GUERRIERI, ROBERTO
2011
Abstract
We present the development and application of a microfluidic device that can efficiently and reproducibly isolate a programmable number of cells into microwells. The chip consists of a 3x4 array of 100 µm microwells drilled through a flex-PCB substrate along microchannels. Negative DEP focusing of particles is used to axially centre particles within the channel and also to shield the microwell entrance for flowing particles. An optical detection routine analyses and selects the cells to be delivered with single cell resolution. Cell viability results based on a calcein release assay performed on single cells are within the range of physiological loss of fluorescence intensity.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
