Single-cell RNA-sequencing revolutionized our approach of transcriptomic studies, enabling to analyze gene expression across cell type in a tissue. Here we introduce an optimized cell dissociation and a Split Pool Ligation-based Transcriptome sequencing (SPLiT-seq) protocol to perform single-cell RNA-seq in insects. Up to 400,000 cells can be used as starting material within a single experiment.
Devilliers, J., Emili, E., Vanni, V., Solana, J., Feuda, R. (2025). An Optimized SPLiT-Seq Protocol for Insects. N/A : Humana Press Inc. [10.1007/978-1-0716-4583-3_11].
An Optimized SPLiT-Seq Protocol for Insects
Feuda R.
Ultimo
Conceptualization
2025
Abstract
Single-cell RNA-sequencing revolutionized our approach of transcriptomic studies, enabling to analyze gene expression across cell type in a tissue. Here we introduce an optimized cell dissociation and a Split Pool Ligation-based Transcriptome sequencing (SPLiT-seq) protocol to perform single-cell RNA-seq in insects. Up to 400,000 cells can be used as starting material within a single experiment.File in questo prodotto:
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