A controlled, blinded and randomized study was performed on an Italian 1500-sow farrow-to-weaning farm. The objective of this evaluation was to assess the correlation of sows and offspring ELISA antibody titres. Twenty-four sows selected to be representative for parity and genetic characteristics of breeding were randomly allocated to 2 groups (group A: 12 sows, group B: 12 sows). Sows in group A received 2 ml Circovac® i.m. 6 weeks and 3 weeks before delivery. Subjects in group B were kept as unvaccinated controls. From litters of these sows was randomly selected a sample of about 3 subjects per litter (group C, piglets born to sows vaccinated: 31 animals; group D, piglets born to unvaccinated sow: 34 subjects). Blood samples were collected from sows 10 days after delivery and from piglets at 10 days of age. The sera were tested for antibodies against PCV2 by ELISA (Serelisa®PCV2 Ab, Synbiotics). Animals were assigned into 4 categories based on the median of log10 ELISA titre: low titre sows (<3.74), high titre sows (>=3.73); low titre piglets (<3.91), high titre piglets (>=3.91). Percent agreement of low sows-low piglets and high sows-high piglets titres [(30+26)/65] was 86% with a Kappa of 0.72 (substantial agreement). Vaccination of the sows provides high maternal antibody titres in piglets with a substantial agreement between high sows-high piglets PCV2 titres.
Ostanello F., Granito G., Rugna G., Lelli D., Leotti G., Merialdi G., et al. (2011). Agreement of dam and piglet PCV2 ELISA titres. s.l : s.n.
Agreement of dam and piglet PCV2 ELISA titres
OSTANELLO, FABIO;
2011
Abstract
A controlled, blinded and randomized study was performed on an Italian 1500-sow farrow-to-weaning farm. The objective of this evaluation was to assess the correlation of sows and offspring ELISA antibody titres. Twenty-four sows selected to be representative for parity and genetic characteristics of breeding were randomly allocated to 2 groups (group A: 12 sows, group B: 12 sows). Sows in group A received 2 ml Circovac® i.m. 6 weeks and 3 weeks before delivery. Subjects in group B were kept as unvaccinated controls. From litters of these sows was randomly selected a sample of about 3 subjects per litter (group C, piglets born to sows vaccinated: 31 animals; group D, piglets born to unvaccinated sow: 34 subjects). Blood samples were collected from sows 10 days after delivery and from piglets at 10 days of age. The sera were tested for antibodies against PCV2 by ELISA (Serelisa®PCV2 Ab, Synbiotics). Animals were assigned into 4 categories based on the median of log10 ELISA titre: low titre sows (<3.74), high titre sows (>=3.73); low titre piglets (<3.91), high titre piglets (>=3.91). Percent agreement of low sows-low piglets and high sows-high piglets titres [(30+26)/65] was 86% with a Kappa of 0.72 (substantial agreement). Vaccination of the sows provides high maternal antibody titres in piglets with a substantial agreement between high sows-high piglets PCV2 titres.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
