Cultured organotypic hippocampal slices (hOTCs) have become increasingly popular as a model for studying brain function. This model offers significant advantages over traditional in vitro methods, as they allow the examination of mid to long-term manipulations while preserving the structure of the dentate gyrus (DG) in the hippocampus. In this chapter, we focus on a protocol based on hOTCs of mouse entorhinal cortex and hippocampus, which by integrating techniques such as retroviral injections, immunohistochemistry, and microscopy imaging, physiological or pathological processes can be easily investigated.
Rodríguez-Bodero, A., Bonifazi, P., Tønnesen, J., Encinas-Pérez, J.M. (2025). Picrotoxin-Induced Epileptogenic Hippocampal Organotypic Slice Cultures (hOTCs). londra : Humana Press Inc. [10.1007/978-1-0716-4386-0_23].
Picrotoxin-Induced Epileptogenic Hippocampal Organotypic Slice Cultures (hOTCs)
Bonifazi, Paolo;
2025
Abstract
Cultured organotypic hippocampal slices (hOTCs) have become increasingly popular as a model for studying brain function. This model offers significant advantages over traditional in vitro methods, as they allow the examination of mid to long-term manipulations while preserving the structure of the dentate gyrus (DG) in the hippocampus. In this chapter, we focus on a protocol based on hOTCs of mouse entorhinal cortex and hippocampus, which by integrating techniques such as retroviral injections, immunohistochemistry, and microscopy imaging, physiological or pathological processes can be easily investigated.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
